摘要
使用大庆油田石油污染土壤中分离出优势菌种(革兰氏阴性G-、黄杆菌属Flavobacterium),在实验室可控条件下,研究了该菌种对苯的降解规律和特点.研究发现:微生物对苯浓度耐受范围8.8～17.6 mg·L-1,大于17.6mg·L-1时,对该菌株产生明显的抑制作用.降解体系在pH为6.5～7.0之间达到对苯的较高降解水平,最佳降解率出现在苯初始浓度7.04～13.2mg·L-1之间;苯在微生物细胞内外的浓度变化趋势呈现一致.-lgP(P为有机溶剂苯在细胞膜和水相中的分配系数的比值)的变化能够较好的表征苯在微生物细胞内外的降解趋势和毒性变化;当体系中苯的初始浓度大于8.8mg·L-1时,苯的降解率与P值变化趋于一致.
The experiments were made in laboratory to analyze the characteristics and principle of benzene＇s biodegradation using the microorganism （G^- , Flavobacterium ） taken from Daqing oil fields. Re.suits show that the maximal concentration of benzene, which microorganisms could endure is between 8.8 mg·L^-1 and 17.6mg·L^-1 ; microorganisms were inhibited as benzene＇s concentration was beyond 17.6mg·L^-1. Trends of benzene＇s concentration in and out of microorganism＇s cell are almost same; the biodegradation could be achieved efficiently as the pH range of 6.5- 7.0 and benzene initial concentration range of 7.04--13.2mg·L^-1. Change of -IgP（P is the distributive ratio of benzene in membrane and water） could be illustrated the trends of toxicity and degradation of benzene in and out of microorganism＇s cell ; the biodegradation rate varied simultaneously with the change of P as initial concentration of benzene was beyond 8.8mg·L^-1.
出处
《环境科学》
EI
CAS
CSCD
北大核心
2005年第6期148-152,共5页
Environmental Science
基金
国家自然科学基金项目(40472129)
