摘要
目的探讨乌司他丁在大鼠胰岛分离中的保护作用。方法用含胶原酶的Hanks溶液灌注大鼠胰腺。根据胶原酶中乌司他丁的含量分为3组。低浓度组:胶原酶中含乌司他丁2500U/ml;高浓度组:胶原酶中含乌司他丁5000U/ml;对照组:胶原酶中不含乌司他丁。将各组分离出的胰岛进行比较。结果加用乌司他丁的两组分离出的胰岛在形态、数量方面均优于对照组(P<0.01)。在无糖、低糖、高糖和茶碱+高糖刺激下,加用乌司他丁的两组胰岛素的释放量优于对照组(P<0.01)。结论用乌司他丁灌注胰腺对大鼠胰岛分离具有保护作用。
Objective To observe the protective effect of Ulinastatin in rat islet isolation and advance an improved method in rat pancreas islet isolation. Methods Rat pancreas was perfused with Hanks solution containing collagenase. The rats were divided into 3 group according the Ulinastatin concentrations in collagense, low-concentration Ulinastatin （2500 U/ml） group, high-concentration Ulinastatin （5000 U/ml） and control group （no Ulinastatin）. The isolated pancreases were compared among the groups. Results The shape and number of the isolated pancreases in low-concentration Ulinastatin and high-concentration Ulinastatin groups were superior to those in control group （P〈0.01）. Under the stimuli of no glucose, low glucose, high glucose and theophylline with high glucose, the amount of insulin release in low-concentration Ulinastatin and high-concentration Ulinastatin groups was greater than in control group （P〈0.01）. Conclusion Ulinastatin perfusion can protect the islet from trypsin digestion in the process of rat islet separation.
出处
《中华器官移植杂志》
CAS
CSCD
北大核心
2005年第12期732-734,共3页
Chinese Journal of Organ Transplantation
基金
黑龙江省自然科学基金项目资助(D0118)
关键词
细胞分离
胰岛
胰蛋白酶抑制剂
大鼠
Cell separation
Islet of Langerhans
Trypsin inhibitors
Rat
