摘要
目的:研究集落刺激因子(colony-stimulating factor-1,CSF-1)和甲状旁腺相关蛋白(parathyroid hormone-related protein,PTHrP)对体外培养的人牙囊细胞(dental follicle cells,DFC)表达骨保护素(osteoprotegerin,OPG)的影响.方法:原代培养人牙囊细胞,选第3～6代细胞,培养基中分别加入不同浓度的CSF-1和PTHrP与牙囊细胞共同孵育,MTT法检测人牙囊细胞的增殖活性;Sandwich ELISA测定培养上清液中OPG的含量.结果:25ng/ml的CSF-1和10 ng/ml的PTHrP可以促进DFC增殖,并且此浓度两种因子分别与DFC共培养OPG表达均降低(P＜0.05).结论:在牙齿萌出的某时期牙囊自分泌和旁分泌的CSF-1和PTHrP有降低人牙囊细胞OPG表达的作用.
Objective.. To study the effects of, colony-stimulating factor-1 （CSF-1）and parathyroid hormone-related protein（PTHrP） on the secretion of osteoprotegerin （OPG）in human dental follicle cells. Method： Human dental follicle cells culture was established , and the third- to sixth- passage cells were incubation with CSF-1 or PTHrP for both timeand dose- dependent studies. MTT assays were used to evaluate the cells proliferation and enzyme-linked immunosorbent assays were used to measure the amount of OPG in the conditioned mediums. Result：This study showed that proliferation activity increased and the secretion of OPG decreased significantly after incubation of the human dental follicle cells with 25 ng/ml CSF-1 and 10 ng/mlPTHrP. Conclusion：CSF-1 and PTHrP can decrease the expression of OPG of human dental follicle cells at some stage during tooth eruption.
出处
《临床口腔医学杂志》
2005年第12期709-712,共4页
Journal of Clinical Stomatology
基金
国家自然科学基金资助项目(30400510)
关键词
牙囊
集落刺激因子
甲状旁腺相关蛋白
骨保护素
牙齿萌出
dental follicle
colony-stimulating factor-1
parathyroid hormone-related proteim osteoprotegerin
tooth eruption
