摘要
探讨不同的治疗方法对急性重症胆管炎并发肺损伤的影响及治疗机制。采用＾３５Ｓ标记大肠杆菌为示踪物观察肠道细菌易位，＾１２５Ｉ标记的牛血清白蛋白测定肺微血管通透性；电子自旋共振仪测定组织中氧自由基含量；生化方法测定组织中磷脂酶Ａ２（ＰＬＡ２）活性；结果：ＡＣＳＴ时肠道内细菌可通过门静脉向肺脏大量易位，诱发肺功能损伤。
In this study,we observed the bacterial translocationusing (35)~S labelled E. coli as tagged material. Lungpermeability was measured with bovine serum albuminlabelled with (125)~I ;oxygen free radicals (OFR)weredetermined with electron spin resonance spectrometer;and, the activity of phospholipase A_2 (PLA_2 ) was assessedby using biochemical method. The results demonstratedE. coli could translocate to the lung fromthe gut obviously via portal vein which induced a seriouslung injury in the presence of acute cholangitis ofsevere type (ACST ). Lung permeability increased obviouslyin ACST rats. The changes of OFR and PLA_2parelleled the lung injury. Among the three therapies,only Chinese herbs presented an effective protection.Tissue injury was not altered significantly by the internaldrainage of the bile duct or by using ampicillin,and,they seemed even to aggravate tissue injury. Theeffects of different treatments on the pulmonary functionwere closely related to their effects on the bacterialtranslocation and the OFR and PLA_2 in lung tissues.
出处
《中国危重病急救医学》
CAS
CSCD
1996年第7期386-389,共4页
Chinese Critical Care Medicine
基金
国家中医药管理局科研资助
关键词
胆管炎
肺损伤
细菌易位
氧自由基
磷脂酶A2
acute cholangitis of severe type
lung injury
bacterial translocation
oxygen free radicals
phospholipase A_2
