摘要
Metabolism of pilocarpine in human urine was studied by high-performance liquid chromatography with tandem mass spectrometric method after single oral dose 10 mg pilocarpine hydrochloride to a healthy male volunteer.The urine sample was collected from 0 to 8 h after oral administration. The urine sample was prepared by solid phase extraction,and then was analyzed using high-performance liquid chromatography coupled with a triple quadrupole tandem mass spectrometer in multiple reaction monitoring and product ion scan mode. Three metabolites were identified as the oxidative metabolite,pilocarpic acid and the glucuronide of pilocarpine.
Metabolism of pilocarpine in human urine was studied by high-performance liquid chromatography with tandem mass spectrometric method after single oral dose 10 mg pilocarpine hydrochloride to a healthy male volunteer. The urine sample was collected from 0 to 8 h after oral administration. The urine sample was prepared by solid phase extraction, and then was analyzed using high-performance liquid chromatography coupled with a triple quadrupole tandem mass spectrometer in multiple reaction monitoring and product ion scan mode. Three metabolites were identified as the oxidative metabolite, pilocarpic acid and the glucuronide of pilocarpine.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2005年第12期2230-2232,共3页
Chemical Journal of Chinese Universities
基金
国家自然科学基金(批准号:20471021)资助
关键词
毛果芸香碱
代谢物
液相色谱-串联质谱法
Pilocarpine
Metabolite
Liquid chromatography-tandem mass spectrometry
