摘要
以超级杂交中稻恢复系9311为受体和轮回亲本,马来西亚普通野生稻(O.rufipogon)为增产QTLyld1.1和yld2.1的供体进行杂交和连续回交,各世代采用分子标记辅助选择,至BC6F1后自交,得到BC6F2群体,通过分子标记检测,获得分别携带野生稻增产QTLyld1.1y、ld2.1及同时携带yld1.1和yld2.1的3套近等基因系。对同时携带yld1.1和yld2.1的近等基因系进行遗传背景分析,发现其与受体9311的遗传组成有93.9%一致。田间试验表明,野生稻增产QTL近等基因系的产量均高于受体,说明将野生稻增产QTL转移至杂交稻恢复系中,能提高其产量水平,且2个QTL的增产效果大于单个QTL的效果。
Using 9311, a restorer line of two-line super-hybrid rice, as the recipient and recurrent parent and Oryza ruffpogon as the donor of two yield-enhancing QTL alleles, yldl. 1 and yld2.1 on chromosomes 1 and 2, respectively, three types of near-isogenic lines （NIL） containing yldl. 1 and yld2.1 or yldl. 1 or yld2.1 were developed by successive backcrossing and molecular marker-assisted selection （MAS）. The genetic background of the near-isogenic lines was similar to that of the original parent 9311 at a degree of 93.9%. The NILs with the yield-enhancing QTLs had a higher grain yield than 9311 in the field experiments, which indicates that transferring the yield-enhancing QTLs into the restorer line of hybrid rice can increase its yielding ability. Furthermore, the yield-increasing effect of the NILs with both yldl. l and yld2. l was greater than that of the NILs with only a single OTL allele yldl. 1 or yld2.1.
出处
《杂交水稻》
CSCD
北大核心
2005年第6期52-56,共5页
Hybrid Rice
基金
国家自然科学基金项目(30270819)
国家"863"计划项目(2004AA211142)
关键词
水稻
野生稻增产QTL
分子标记辅助选择(MAS)
近等基因系
rice
yield-enhancing QTLs
wild rice （ O. rufipogon ）
marker-assisted selection （MAb）
near-isogenic lines （NILs）
