摘要
目的建立一种测定血清中1,5-脱水葡萄糖醇(1,5-AG)的方法,辅助诊断糖尿病.方法经葡萄糖激酶(GK)和葡萄糖-6-磷酸脱氢酶(G6PD)偶联系统使血清中葡萄糖彻底转化为不与呋喃糖氧化酶(PROD)反应的6-磷酸葡萄糖酸内酯,以PROD氧化1,5-AG,生成1,5-脱水果糖和H2O2,后者用Trinders反应进行比色测定.结果该方法在550 μmol/L以内线性良好.高值(455.5 μmol/L)、中值(215.8 μmol/L)和低值(15.1 μmol/L)批内变异系数(CV)分别为0.61%、0.84%和1.70%,批间CV分别为1.12%、2.00%和2.70%.平均回收率为101.6%.本法与微柱法相关性良好(r=0.999 8).用本法对100名健康者测定1,5-AG水平为(161.9±40.2) μmol/L,呈正态分布.参考值范围((-x)±1.96s)为83.1～240.7 μmol/L,无性别、年龄差异.结论该法简便、快速、灵敏、准确和稳定,可用于糖尿病的及时诊断与监控,适合于各类实验室常规应用.
Objective To establish a new method for measuring serum 1, 5- anhydro-D-glucitol （1,5-AG）. Methods Glucokinase（GK） and glucose-6-phosphate dehydrogenase （G6PD） were used to avoid interference of internal glucose in serum sample, and pyranose oxidase （PROD） was used to oxidize 1,5-AG with the formation of 1,5-anhydro fructose and H2O2. Measurement was done according to the Trinders reaction principle. Results The linear range was good within 550 μmol/L. With high, medial, and low concentrations, the CVs of within run were 0. 61%, 0. 84% and 1.70%, and between run were 1.12%, 2.00% and 2.70% respectively. The average recovery was 101.6%. Results of this method correlated well with that of tiny-columnination. The level of 1, 5-AG in control group was （ 161.9 ±40.2） μ mol/L, reference range （x^- ＋ 1.96s） was 83.1±240.7 μ mol/L, and no difference in age and sex. Conclusions This method is easy, rapid and precise to diagnose and monitor diabetes mellitus.
出处
《检验医学》
CAS
北大核心
2005年第6期511-514,共4页
Laboratory Medicine
