摘要
应用免疫胶金技术证实钩端螺旋体脂多糖（Ｌ－ＬＰＳ）作用于体外培养的小鼠成纤维细胞后５ｍｉｎ即能侵入胞浆，３０ｍｉｎ后可在细胞核中检出Ｌ．一！Ｉ５。钩端螺旋体实验感染的豚鼠各内脏组织的细胞间隙、胞浆及核等内均存在１．一！，ＰＳ，尤以肾和肺脏检出阳性率较高。Ｌ－ＬＰＳ检测阳性的小鼠成纤维细胞和感染豚鼠内脏组织细胞均出现相似的超微结构病变，如内质网扩张、膜旁核糖体脱落、线粒体嵴或外膜消失等。实验结果表明Ｌ－ＬＰＳ是钩端螺旋体主要致病物质之一。
Leptospiral lipopolysaccharides(L-LPS)could invase the cytoplasm in 5 minutes and it could be detected from the nucleus of mouse fibroblasts in vitro in 30 minntes by immuno-aurosol technique.L-LPS Positive rates in the samlples of lungs and kidneys of infected guinea pigs were higher than that in the intercellular space,cytoplasm and nucleus of the other internl organs.There were simil ultrastructure changes in mouse fibroblasts or in the cells of internal organs of guinea pigs in which L-LPS examinations were positive.the ultra-structure changes included dilatation of endoplasmic reticulum,exfoliation of paramembeanous ribosome particles,disappearance of mitochondrial crista and outer mitochondrial membrane.The results directly proved that L-LPS was one of the important pathogenic substances of leptospira.
出处
《中国人兽共患病杂志》
CSCD
北大核心
1996年第4期15-17,共3页
Chinese Journal of Zoonoses
基金
浙江省自然科学基金
关键词
钩端螺旋体
脂多糖
成纤维细胞
Leptosoira
Lipopolysaccharides
Invasion
Location
UItrastructure Pathologic
Change.
