摘要
目的探讨环氧合酶2(COX2)和黏附分子在再灌注后炎症损伤中的作用。方法线栓法制作大鼠大脑中动脉缺血模型(MCAO)。用免疫印迹及免疫组化方法观察再灌注后不同时间脑组织细胞间黏附分子1(ICAM1)、E选择素及COX2的表达。白细胞髓过氧化物酶(MPO)检测试剂盒检测MPO活性。结果假手术组及再灌注4、22、46h组,缺血脑皮质及纹状体中COX2含量相对灰度值分别为0.7642±0.0763、1.5382±0.1047、1.6491±0.3265、1.8020±0.3719及0.7104±0.0891、2.2061±0.2143、1.7897±0.3537、1.8018±0.5703;ICAM1相对灰度值分别为0.6845±0.0531、0.9115±0.0422、0.9426±0.0407、1.0756±0.0467及0.6583±0.0361、0.9439±0.0746、0.9975±0.1532、0.8808±0.0497。再灌注各组MPO活性、COX2、ICAM1和E选择素表达均较假手术组高。免疫组化显示,缺血再灌注后,缺血边缘区出现大量E选择素和ICAM1免疫染色阳性的微血管,在皮质的COX2阳性细胞主要为神经元,纹状体出现了免疫阳性的胶质细胞和血管内皮细胞。再灌注后不同时间皮质COX2与ICAM1表达量呈正相关(n=6,r=0.973,P<0.05);纹状体COX2与E选择素表达量呈正相关(n=6,r=0.958,P<0.05)。结论炎症反应参与了缺血周边区的再灌注后继发性损伤,COX2和内皮黏附分子的表达都在再灌注后炎症反应中发挥重要的作用。
Objective To clarify the possible role of cyclooxygenase-2 （COX-2）, intercellular adhesion moleeule-1 （ICAM-1） and E-seleetin in the inflammatory injury indueed by eerebral isehemia and reperfusion. Methods The focal cerebral ischemia and reperfusion model was induced by a suture occlusion of the right middle cerebral artery. The rats were randomly assigned to four groups： sham operated group; 4 h,22 h and 46 h reperfusion groups. The expression of ICAM-1, E-selectin and COX-2 were detected by immuno-Western blot and immunohistochemical method. Myeloperoxidase （MPO） activity was detected by a commercial MPO kit. Results In the fight cortex and striatum of the sham operated group and the 4 h,22 h and 46 h reperfusion groups, the relative values of COX-2 were 0. 7642 ± 0. 0763, 1. 5382 ± 0. 1047, 1. 6491 ± 0. 3265, 1. 8020 ± 0. 3719 and 0. 7104 ± 0. 0891, 2. 2061 ± 0. 2143, 1. 7897 ± 0. 3537, 1. 8018 ± 0. 5703 respectively; the relative values of ICAM-1 were 0. 6845 ± 0. 0531, 0. 9115 ± 0. 0422, 0. 9426 ± 0.0407, 1.0756 ±0.0467 and 0.6583 ±0.0361, 0.9439 ±0.0746, 0.9975 ±0. 1532, 0. 8808±0.0497 respectively. Significant increase of MPO activity and expression levels of COX-2, ICAM-1 and E-selectin were shown from 4 h to 46 h in the striatum and cortex of rats following reperfusion, hnmunohistochemical staining showed that the number of ICAM-1 and E-selectin positive vessels was increased in the border of ischemia. The positive cells of COX-2 were almost neurons in the cortex, but in the striatum, the cells were neurons, glias and vessel endothelium. Positive eorrelation between COX-2 and ICAM-1 expression in the cortex （ n = 6, r = 0. 973,P 〈 0.05 ） was noted, and COX-2 and E-selectin in the striatum as well （ n = 6, r = 0. 958 ,P 〈 0.05）. Conclusions Inflammatory reaction might be involved in secondary brain injury after cerebral ischemia and reperfusion. The adhesion molecules and COX-2 should be the same important roles in this process.
出处
《中华神经科杂志》
CAS
CSCD
北大核心
2005年第10期632-635,共4页
Chinese Journal of Neurology
关键词
脑缺血
再灌注
胞间黏附分子1
前列腺素内过氧化物合酶
Brain ischemia
Reperfusion
Intercellular adhesion molecule-l
Prostaglandin-endoperoxide synthase
