摘要
目的观察重组中国人γ干扰素(INF-γ)对博莱霉素(BLM)所致小鼠肺损伤(PI)的防治作用。方法75只C57小鼠随机分为对照组、BLM组、BLM+INF-γ小剂量组(0.25μg/d)、BLM+INF-γ中剂量组(0.5μg/d)、BLM+INF-γ大剂量组(1.0μg/d),每组15只。实验组小鼠以BLM诱导PI,分别用不同剂量的INF-γ进行防治。对照组小鼠不进行任何处理。实验结束后处死小鼠分别进行肺羟脯氨酸(hydroxyproline,Hyp)测定,用图像分析法计算Ⅰ、Ⅲ型胶原面积与切片面积,肺泡面积与肺视野面积比。结果对照组小鼠肺组织Hyp为(0.65±0.06)μg/mg、BLM组为(0.78±0.08)μg/mg,两者比较差异有统计学意义(P<0.01);对照组Ⅰ、Ⅲ型胶原为(0.004±0.001)、BLM组为(0.048±0.006),两者比较差异有统计学意义(P<0.01);BLM+INF-γ大剂量组大鼠肺组织Hyp为(0.67±0.08)、Ⅰ、Ⅲ型胶原含量为(0.008±0.001)与对照组相比差异均有统计学意义(P均<0.01);各组肺泡与肺组织面积比随着INF-γ的剂量增加而增高,分别为1.78、0.12、0.67、0.73、1.65。结论INF-γ能有效抑制成纤维细胞向肌成纤维细胞转化,减少肺内胶原合成,对PI有一定防治作用。
Objective To observe the protective role of recombinant Chinese interferon-γ （INF-γ） in pulmonary injury （PI） induced by bleomycin （BLM） in C57 mice. Methods Seventy-five C57 mice were randomly divided into a control group and groups treated with BLM, BLM ＋ INF-γ minimum dose （0. 25 μg/d）, BLM ＋ INF-γ medium dose （0. 5 μg/d）, and BLM ＋ INF-γ maximum dose （ 1.0 μg/d, with 15 mice each）. PI was induced by BLM, and intervention with different doses of INF-γ was carried out in the experiment groups, but no treatment was administered in the control mice. Measurement of pulmonary hydroxyproline （Hyp）, image analysis of collagen Ⅰ and Ⅲ, and measurement of the ratio between lung alveoli and interstitial areas were performed. Results Lung Hyp content and collagen Ⅰ and Ⅲ deposition were increased as compared with the control after administration of BLM （0. 78 ± 0. 08 vs 0. 65 ± 0. 06, P 〈0. 01 ; 0. 048 ±0. 006 vs 0. 004 ± 0. 001, P 〈0. 01 ）. Hyp content and collagen Ⅰ and Ⅲ deposition were reduced in group INF-γ maximum dose （0. 67 ± 0. 08 vs 0. 78 ±0. 08, P 〈 0. 05 ; 0. 008 ± 0. 001 vs 0. 048 ± 0. 006, P 〈0. 01 ）. The ratio of lung collagen to lung tissue areas was increased with the increase of INF-γ doses （1.78, 0. 12, 0. 67, 0. 73, 1.65 respectively）. Conclusion INF-γ is effective in alleviating BLM induced pulmonary injury in mice, possibly by inhibition of transformation of fibroblasts to myofibroblasts and collagen synthesis.
出处
《中华结核和呼吸杂志》
CAS
CSCD
北大核心
2005年第11期760-762,共3页
Chinese Journal of Tuberculosis and Respiratory Diseases
基金
军事医学科学研究基金资助项目(2002)
关键词
干扰素γ
重组
小鼠
肺纤维化
动物实验替代实验
治疗
Interferon-gamma, recombinant
Mice
Pulmonary fibrosis
Animal testingahemative
Therapy
