摘要
目的:检测水通道蛋白(AQP)-1,3在豚鼠耳蜗及内淋巴囊中的表达情况。方法:运用免疫组织化学二步法及免疫荧光染色检测豚鼠耳蜗及内淋巴囊中AQP-1,3的表达。结果:AQP-1主要表达于耳蜗螺旋韧带基底部、Corti器基底膜及鼓阶内侧上皮面及内淋巴囊上皮细胞下的基质组织中;AQP-3主要表达于血管纹、螺旋韧带、Corti器、螺旋神经节细胞及内淋巴囊的上皮细胞及其下的基质成分中。结论:AQP-1,3在豚鼠耳蜗及内淋巴囊中存在广泛表达,但其功能仍未明确。
Objective： To detect the expression of Aquaporin 1,3 in the cochlea and endolymphatic sac of guinea pig. Method：Two-step immunohistochemical method and immunofluorescence was used to examine the expression of Aquaporin-1,3. Result： Aquaporin-1 was expressed on the basilar part of spiral ligament, basal mem brane of Corti＇s organ and epithelialis of scala tympani, and basilar part under the cellula epithelialis in endolym phatic sac. Aquaporin 3 was expressed on stria vascularis, spiral ligament, Corti＇s organ, spiral ganglionand, the basilar part and the cellula epithelialis in endolymphatic sac. Conclusion： Aquaporin-1,3 were widely expressed in the cochlea and endolymphatic sac of guinea pig.
出处
《临床耳鼻咽喉科杂志》
CSCD
北大核心
2005年第23期1085-1087,共3页
Journal of Clinical Otorhinolaryngology
