摘要
目的:建立和评价海人酸杏仁核微量注射点燃癫痫动物模型。方法:选用雄性Wistar大鼠,以杏仁核外侧基底核为给药靶点,在立体定位仪下,微量注射海人酸1μg后进行大鼠的行为学和电生理学观察,并分别于第6、12、72 h和7、14、21 d对大鼠脑组织进行病理学观察。结果:海人酸微量注射后实验大鼠出现典型的癫痫发作过程,顶叶皮层脑电图依次出现多种形式的痫性放电,HE染色显示海马和颞叶皮层神经细胞的相应病理变化,点燃成功率为90%。结论:采用杏仁核海人酸微量注射方法成功建立了Wistar大鼠点燃癫痫模型,该模型适用于颞叶癫痫的相关研究。
Objective To establish and evaluate epileptic model kindled by kainic acid microinjected into nucleus amygdalae. Methods Kainic acid was microinjected （1 μg） into right nucleus amygdalae of Wistar male rats with stereotaxic technique. Praxiology and electrophysiology observation were performed after kainic acid injection, as well as pathological detection on 6th, 12th, 72th hour and 7th, 14th, 21st day, respectively. Results The experimental rats presented typical epileptic seizures relapse and various epileptic discharge in parietal cortex electroenciphalogram. The nerve cell damage was observed through HE staining in hippocampus, or temporal lobe. The kindling rate of the model was 90%. Conclusion The epileptic model has been successfully established by kainic acid microinjected into nucleus amygdalae in Wistar male rats, this model is applicable for temporal lobe epilepsy.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2005年第6期858-861,F0002,共5页
Journal of Jilin University：Medicine Edition
基金
吉林省科技厅科技发展项目资助课题(20030430)
长春市科技发展项目资助课题(176s15)
关键词
癫痫
颞叶
海人酸
点燃效应
模型
动物
epilepsy, temporal lobe
kainic acid
kindling
model, animal
