摘要
目的研究乙型肝炎病毒(HBV)血清标志物定量与HBV DNA定量及乙型肝炎病毒表面抗原蛋白前S1(PreS1)的关系。结果用时间分辨荧光免疫分析技术(TRFIA)对乙型肝炎五项定量分析。用荧光定量(FQ)-聚合酶链反应(PCR)技术对HBV DNA定量分析。用酶联免疫吸附试验(ELISA)测定PreS1。结果HBVDNA拷贝数与乙型肝炎表面抗原(HBsAg)、乙型肝炎e抗原(HBeAg)值呈正相关(相关系数分别是r=0.272、P<0.05;r=0.0054,P<0.01),与乙型肝炎核心抗体(HBcAb)值呈负相关(r=-0.273,P<0.01);HBeAb(+)组的HBV DNA平均拷贝数明显高于HBeAb(+)组;PreS1在HBeAg(+)组阳性率高于抗HBe(+)组。结论HBeAg与HBV DNA有相关性,是病毒复制的指标,两者与PreS1具有伴随关系;HBsAg是HBV外膜的组成部分,它的高表达与HBV复制呈正相关。
Objective ①To quantify the serum HBV markers and to study their clinical significance. ②To check the relationship of HBV DNA with HBV markers in serum so as to provide evidences for early diagnosis,rational drug use and better curative effect to the HBV-infected. Methods ①Time-resolved fluoroimmune assay （TRFIA） was used to measure HBV markers in serum. ②Fluoroimmune quantitative PCR（FQ-PCR） was used to measure HBV DNA. Results There was a direct correlation between quantitafions of HBV DNA and HBeAg or HBsAg, a negative correlation existed between quantitations of HBV DNA and HBcAb. Mean copies of HBV DNA was higher in the group of HBeAg（＋） than in the group of HBeAb（＋）; PreS1 pesifivity of the HBeAg（＋） group was higher than that of the anti-HBe（＋） group. Conclusion HBeAg has correlation with HBV DNA, being a marker of virus replication; HBeAg, HBV DNA and PreS1 have always been in accompaniment; HBsAg is a part of HBV adventitia, its high expression positively correlates with HBV DNA＇s replication.
出处
《中国药物与临床》
CAS
2005年第11期837-839,共3页
Chinese Remedies & Clinics
