摘要
目的探讨磁共振对超顺磁性氧化铁(SPIO)氨基硅烷Fe3O4纳米颗粒体外标记人肺腺癌(SPC-A-1)进行成像的可行性。方法制备氨基硅烷Fe3O4纳米颗粒,分别培养肺腺癌细胞株SPC-A-1及人胚肺细胞株WI38至对数生长期,更换为分散有氨基硅烷Fe3O4纳米颗粒的培养液,经培养1、3、6 h,分别取所培养的细胞进行电镜超微结构观察和磁共振T1WI、T2WI和FGR/30°三个序列对培养6 h的细胞群成像。结果同一条件下培养6 h,见有多量氨基硅烷Fe3O4纳米颗粒进入肺腺癌细胞SPC-A-1的细胞质内,而WI38人胚肺细胞未见氨基硅烷Fe3O4纳米颗粒进入。对培养6 h的细胞群磁共振成像中,标记后的肺腺癌细胞在T1WI、T2WI和FGR/30°三个序列中均可显示SPIO信号,其中FGR/30°序列信号强度变化率最大。结论氨基硅烷Fe3O4纳米颗粒可以标记SPC-A-1人肺腺癌细胞,应用磁共振可以对其进行监测。
Objective To study the feasibility of MR imaging for labeled human lung adenocarcinoma cell line （SPC-A-1） with ultrasmall superparamagnetic iron oxide （SPIO） particles in vitro. Methods Both human lung adenocarcinoma cell line SPC-A-I and human lung embryo cells WI38 were cultured respectively in medium contained SPIO （aminosilane-coated nano Fe3O4 particles）. From one to six hours, the ultrastructure of both cells was observed by transmission electron microscopy to determine particles uptake and their distribution in cells. The cells underwent MR imaging with TlWI, T2WI and FGR/ 30° sequences. Results Human lung adenocarcinoma cell line SPC-A-I have taken up a lot of SPIO particles within the first 6 hours, while normal human lung embryo cells WI38 do not take up any nanoparticles under the same conditions. The signal intensity decrease for the labeled cells compared with that for unlabeled cells was demonstrated on TlWl, T2Wl and FGR/ 30° sequences. The percentage change in FGR/30°sequence was the largest. The percentage change in signal intensity was larger for 1 × 10^8 labeled cells than that for 5 × 10^7 labeled cells. Conclusion Human lung adenocarcinoma cell line SPC-A-I can be labeled with SPIO particles, and the labeled cells can be imaged with 1.5T MR equipment.
出处
《中国医学影像技术》
CSCD
北大核心
2005年第11期1652-1654,共3页
Chinese Journal of Medical Imaging Technology
基金
国家自然科学基金资助(30170286)
关键词
磁共振成像
肺肿瘤
腺癌
超顺磁性氧化铁
Magnetic resonance imaging
I.ung neoplasms
Adenocarcinoma
Superparamagnetic iron oxide
