摘要
目的:研究泌乳素(PRL)腺瘤中垂体瘤转化基因(PTTG)和抑癌基因PTEN在泌乳素腺瘤中的表达及意义。方法:52例泌乳素腺瘤分为侵袭组24例和非侵袭组28例,采用免疫组化方法分别检测腺瘤组织中PTTG蛋白和PTEN蛋白的表达水平;应用逆转录聚合酶链反应(RT-PCR)检测腺瘤组织中PTTG-mRNA的表达水平;应用免疫印迹(Western-blot)方法检测腺瘤组织中PTTG蛋白的表达水平并进行定量分析。结果:PTTG蛋白在侵袭组中有23例阳性表达,积分光密度(IOD)为9874.24±2143.56,平均吸光度为28.13±4.31,显著高于非侵袭组;RT-PCR检测显示,48例腺瘤标本有PTTG-mRNA特异扩增条带,但侵袭组表达水平有不同程度的增高;PTEN蛋白在非侵袭组中有8例阳性表达,IOD为6489.12±1523.45,与侵袭组比较差异有统计学意义。结论:基因PTTG与PTEN表达与肿瘤的侵袭性密切相关,对肿瘤的发生、发展有重要作用,可以作为临床判定肿瘤侵袭生长的分子标志。
Objective：To investigate expression of gene PTTG, PTEN and their significance in human prolactinomas. Methods：Fifty-two prolactinomas specimens were divided into invasive group and noninvasive group. Expressions of PTTG protein and PTEN protein were examined by immunocytochemical method; PTTG-mRNA was detected by RT-PCR method; Expression of PTTG protein was detected by Western-blot method. Results ：There were 23 cases （23/24） with positive expression of PTTG protein in invasive group, it＇s IOD was 9 874.24±2 143.56 and average absorbency was 28.13±4.31, which were significantly higher than those in non-invasive group; 48 eases （48/52） reported idio-amplification strap of PTTG-mRNA by RT-PCR method, but the expression level increased in invasive prolactinomas; There were 8 cases （8/28） with positive expression of PFEN protein in non-invasive group, it＇s IOD was 6 489.12±1 523.45 and was obviously different with that of invasive prolactinomas. Conclusion：PTTG and PTEN gene in human prolactinomas have important correlation with invasiveness of prolactinomas, and may play important function in prolactinomas. They can serve as the molecule markers of invasive growth in human prolactinomas.
出处
《山东大学学报（医学版）》
CAS
北大核心
2005年第11期1023-1026,共4页
Journal of Shandong University:Health Sciences
