摘要
目的用含血管内皮生长因子(VEGF)的纤维蛋白胶(FG)作用于球囊损伤血管内膜来评估其对血管内皮化、细胞增殖和内膜增生的影响。方法研究FG、VEGF、肝素处理对犬颈动脉内膜损伤的影响。对比FG和FG、VEGF、肝素处理对内膜损伤修复的影响。根据新内膜和中层厚度比值(I/M)及BrdU掺入计算细胞增殖及扫描电镜观察内皮细胞覆盖情况。结果实验组内皮覆盖率术后10d和30d明显高于对照组[(67±31)vs(41±28),(97±10)vs(82±23),均P<0.05];新内膜和中层细胞增殖率术后30d明显增强;损伤血管近段和中段I/M比率30d和90d增加但远段没有变化。仅用FG者也有促进内膜增殖作用(P<0.05),而用FG、VEGF、肝素处理者30d内即可见到损伤血管近段和中段I/M比率增加。结论纤维蛋白胶释放VEGF加肝素能有效加速损伤血管内皮化,但伴有平滑肌细胞增殖和内膜增生。
Objective To study the effect of vascular endothelial growth factor （VEGF） containing fibrin glue （FG） on re-endotheliazation, cell proliferation and intimal hyperplasia in a canine model of carotid artery endothelium injury. Methods The effect of FG/VEGF/heparin versus FG alone treatment was evaluated at the time point of 10, 30, and 90 days by measuring the intima/media （I/M） ratio and cell proliferation by BrdU incorporation using immunohistochernistry. EC coverage was determined by SEM. Results Compared with normal saline control, FG/VEGF/heparin treatment significantly increased EC coverage at day 10 and at day 30 （ P 〈0.05 ） , and cell proliferation rates in the neointima and the media at day 30 and increased the I/M ratio in both proximal and middle but not the distal segments in the 30-day and the 90-day groups. When compared with FG treatment alone, FG/VEGF/heparin treatment had significantly stronger influence on EC coverage at 30 days （P 〈 0. 05）. Conclusions VEGF plus heparin delivered by FG accelerates re-endothelialization, SMC proliferation and intimal hyperplasia.
出处
《中华普通外科杂志》
CSCD
北大核心
2005年第10期663-665,共3页
Chinese Journal of General Surgery
基金
教育部留学回国人员科研启动基金资助项目
