摘要
目的探讨抗孕激素米非司酮对子宫内膜癌细胞周期时相的调控作用.方法体外培养子宫内膜癌HHUA细胞,不同浓度米非司酮处理细胞24～96小时,流式细胞术(FCM)测定癌细胞周期分布的变化;免疫组化法观察细胞周期调控蛋白的表达变化.结果当抗孕激素米非司酮浓度≥5μmol/L作用癌细胞36小时,G1期细胞比率明显上升,S期细胞比率(SPF)降低(P<0.05);细胞周期调控蛋白p21和p16表达明显上调(P<0.05).结论抗孕激素米非司酮通过调节细胞周期相关蛋白表达使HHUA细胞阻滞于G1期,抑制癌细胞增殖.
Objective To study the effect of antiprogestin mifepristone on cell cycle of endometrial carcinoma. Methods Human endometrial carcinoma HHUA cell was cultured in vitro and treated with mifepristone in different concentration for 24-96 hours. Influence of mifepristone on the cell cycle distribution were detected by using flow eytometry （FCM）. The expression of protein related cell cycle was observed by immunohistoehemieal. Results Treated HHUA cell in vitro with mifepristone≥5μmol/L for 36 hours, rate of G1 phase was increased and S-phase fraction （SPF） was decreased obviously （P〈0. I）5）. The expression of cell cycle related protein p21 and p16 were raised significantly （P〈0. 05）. Conclusion Antiprogestins mifepristone could effectively block HHUA cells at G1 phase and inhibit the proliferation by controlling the expression of protein related cell cycle.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2005年第10期634-636,共3页
Cancer Research on Prevention and Treatment
关键词
米非司酮
子宫内膜癌
细胞周期
Mifepristone
Endometrial carcinoma
Cell cycle
