摘要
目的:建立一种方便、实用的慢性器官移植排斥反应模型,为研究器官移植后慢性排斥反应的发病机制、预防和治疗提供平台。方法:以近交系F344大鼠和近交系Lewis大鼠作为供、受体,取供体的双肾作为供肾,借助静脉支架管,行供体的静脉与受体肾静脉的端端吻合,肾动脉与腹主动脉行端侧吻合。施行原位异体肾移植。移植后即给与环孢素腹腔注射。并于术后4周后行病理检查。结果:所有手术均于60min内完成。所有的受体均存活超过60天,围手术期死亡数为零。受体无急性排斥反应发生。4周后可见典型的慢性排斥反应发生。结论:建立了大鼠慢性排斥反应肾移植模型。为研究慢性排斥反应的发生、进展和干预治疗提供了良好的平台。
Objective： To establish an economic and feasible rat renal transplantation model of chronic rejection. Methods： Inbred F344 and Lewis rats were used as the donors and the recipients. Both kidneys were removed from the donors. With the help of PTCA tube, an end-to-end anastomosis of vein was operated .After transplantation,recipients were received ciclosporin every day. Results： All operations were finished within 60 min. No recipients died during operation. No acute rejection evidence was seen. Chronic rejection could be seen obviously 4 weeks after operation. Conclusion： The newly established method is feasible and reliable. The good model of studying chronic rejection were offered.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2005年第11期796-798,共3页
Journal of Nanjing Medical University(Natural Sciences)
基金
国家自然科学基金资助项目(30200264)
关键词
大鼠
肾移植
模型
排斥反应
rats
kidney transplantation
model
rejection
