摘要
背景:白细胞介素1受体拮抗因子能显著减轻神经元的损伤具有神经保护作用。谷氨酸通过激活多种谷氨酸受体导致兴奋毒性作用,同时也可以通过其某些受体发挥神经保护作用。但二者在脑缺血再灌注过程中的联系仍不十分明了。目的:探讨脑缺血再灌注损伤过程中白细胞介素1受体拮抗因子对海马神经元的神经保护作用以及白细胞介素1受体拮抗因子与代谢性谷氨酸受体5之间的关系。设计:完全随机对照实验。单位:中国科学院武汉物理与数学研究所波谱与原子分子物理国家重点实验室,江汉大学医学与生命科学学院。材料:实验于2004-05/2005-01在中国科学院武汉物理与数学研究所波谱学与原子分子物理国家重点实验室完成。选择正常健康纯化Wis-tar大鼠32只。方法:32只大鼠按单纯随机抽样的方法分为正常组、假手术组、生理盐水组和实验组。正常组大鼠不做任何处理,假手术组大鼠仅在颈部前后方做组织分离、椎动脉暴露、颈总动脉游离手术,不结扎和夹闭双侧椎动脉和颈总动脉;生理盐水组大鼠椎动脉用电烧灼的方法永久性阻断熏颈总动脉暂时性穴20min雪夹闭熏夹闭动脉期间内将2μL生理盐水按0.4μL/min的速度注入大鼠一侧穴右侧雪侧脑室熏拔针前滞针5min熏然后松开颈总动脉的动脉夹再灌注2h;实验组大鼠操作过程相同熏仅将生理盐水换成等量的人重组白细胞介素1受体拮抗剂。然后采用苏木精-伊红染色方法对大鼠皮质区域的病理变化进行观察熏免疫组织化学方法对海马神经元代谢性谷氨酸受体5免疫反应性进行观察。主要观察指标:①各组大鼠脑皮质、海马的基本病理学变化。②各组大鼠脑缺血敏感区海马神经元代谢性谷氨酸受体5免疫反应变化。结果:实验组和生理盐水组各有1只在进行缺血再灌注的过程中诱发惊厥被弃用,其余30只大鼠进入结果
BACKGROUND： Interleukin-1 receptor antagonist can relieve damage of neuron and protect nerve. Aminoglutaric acid can induce exitotoxicity through activating some kinds of aminoglutaric acid receptor, at the same time, can protect nerve through some receptors. But the relationship between them was unclear during the process of cerebral ischemia reperfusion. OBJECTIVE： To investigate the effect of neuroprotective actions of interleukin-1 receptor antagonist on hippocampal neurons, and the relationship between interleukin-1 receptor antagonist and metabotropic glutamate receptor 5 within the process of cerebral ischemic reperfusion injury. DESIGN： Completed randomized controlled study. SETTING： State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences; School of Medicine ＆ Life, Jianghan University. MATERIALS： The experiment was performed at State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, Chinese Academy of Science from May 2004 to January 2005. Totally 32 well-being pure breed male Wistar rats were selected. METHODS： Totally 32 rats were divided into four groups by method of the simple random sampling： normal group （n=8） received no surgical treatment, sham operated group （n=8） subjected to only dorsal and ventral neck midline incisions and gently dissection of the bilateral common carotid arteries free of surrounding nerve fibers without occlusion of the both vertebral arteries and common carotid arteries, saline group （n=8） suffered from the permanently occlusion of the both vertebral arteries by electmcauterization and transient （20 minutes） occlusion of bilateral common carotid arteries respectively, and received the treatment of a 2 μL normal saline injection into right lateral ventricle at the rate of 0.4 μL/minute following a needle withdrawal within 5 minutes, and experiment group （n=8） offe
出处
《中国临床康复》
CSCD
北大核心
2005年第40期158-161,F0003,共5页
Chinese Journal of Clinical Rehabilitation
基金
中国科学院"引进海外杰出人才计划"
国家自然科学基金(10234070,30370419)资助项目~~
