摘要
目的用高效液相色谱法测定氨苄西林丙磺舒分散片中的有关物质。方法色谱柱为十八烷基硅烷键合硅胶柱;检测波长232nm;采用A相(含20%磷酸盐缓冲液的乙腈溶液)B相磷酸盐缓冲液[水1mol·L-1磷酸二氢钾溶液1mol·L-1醋酸溶液(909∶10∶1)]为流动相进行梯度洗脱(A相的起始比例为10%,30min内上升至90%);流速为1mL·min-1;柱温为30℃;进样量为10μL。结果本法实现了在较短时间内将原料中引入的有关物质和强力破坏条件下产生的降解产物与主成分之间的基线分离。结论本色谱条件适合氨苄西林丙磺舒分散片中的有关物质的测定。
OBJECTIVE To develop an HPI.C method for determination of related substances in ampicillin and probencid dispers ible tablets. METHODS C18 column with temperature at 30 ℃ was used with a mobile phase of A[phosphate buffer-acetonitrile（20： 80）]-B phosphate buffer [H2O-1 mol·L^-1 KH2 PO4-1 mol·L^-1 HAc（909 ： 10 ： 1 ）1, used a linear gradient of 10% to 90% A phase mixed with B phase in 30 min The elution was performed at the flow rate of 1.0 mL.min 1. UV detective wavelength was 232 nm, the injection volumn was 10μL. RESULTS The better resolution was performed between related substances in short time. CONCLUSION This method is suitable for determination of related substances in ampicillin and probencid dispersible tablets.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2005年第5期445-446,共2页
Chinese Journal of Hospital Pharmacy
