摘要
目的:建立测定异甘草酸镁血药浓度的方法.方法:采用反相高效液相色谱法进行分离、测定.色谱柱采用Hypersil ODS2 C 18 不锈钢柱(300 mm× 4.6 mm,5 μm),流动相为 0.23 mol·L -1 磷酸盐缓冲液(pH 7.4 )-乙腈(79∶21),柱温40 ℃,流速为 1.0 mL·min -1 ,检测波长为250 nm.结果:线性范围为 0.25 ～10 mg·L -1 (r= 0.999 9 )、10～250 mg·L -1 (r= 0.999 3 ),最低检测浓度为 0.1 mg·L -1 ,提取回收率均大于85%.日内及日间精密度均＜10%.结论:本方法简单、快速,灵敏度和准确度较高,能满足异甘草酸镁临床药动学研究的需要.
OBJECTIVE An HPLC method was established to determine the concentration of magnesium isoglycyrrhizinate in human plasma. METHODS Waters HPLC was used with a Hypersil ODS2 C18 （300 mm×4.6mm.5μm） column. The mobile phase was composed of 0. 23mol·L^-1 phosphatic buffer （pH 7. 4）-acetonitrile （79：21）. Flow rate was 1.0 mL·min^-1 and column temperature was maintained at 40 ℃. Detection wavelength was 250 nm. RESULTS A linear relationship was obtained between the peak areas and magnesium isoglycyrrhizinate plasma concentration from 0. 25 to 10 mg·L^-1 （r= 0. 999 9） and also from 10 to 250 mg·L^-1（r = 0. 999 3）. The detection limit was 0. 1 mg·L^-1. The extraction recoveries from plasma were all more than 85%. RSD within day and between days were less than 5.8 % （n = 5 ） and 7. 6 % （n = 5）, respectively. CONCLUSION The method is accurate, stable and simple. It is applicable for the study of pharmacokinetics of magnesium isoglycyrrhizinate in healthy volunteers.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2005年第2期138-139,共2页
Chinese Journal of Hospital Pharmacy
关键词
高效液相色谱法
异甘草酸镁
血药浓度
HPLC
magnesium isoglycyrrhizinate
concentration in humam plasma
