摘要
采用单克隆抗体抑制实验、酶水解法及血小板α－颗粒膜蛋白（ＧＭＰ１４０）检测等技术研究人血小板上中国蕲蛇毒（ＣＡＡＶ）受体的定位。结果发现：抗血小板糖蛋白Ⅰｂ（ＧＰⅠｂ）单克隆抗体ＳＺ－２、ＨＩＰ１显著抑制１２５Ｉ－ＣＡＡＶ和血小板特异性结合并呈浓度依赖性，最大抑制率分别为９４．７％±２．０％和８３．０％±２．９％；随糜蛋白酶水解血小板浓度和作用时间不断增加，血小板膜上的ＧＰⅠｂ不断被酶解去掉，１２５Ｉ－ＣＡＡＶ与血小板特异结合率不断下降，从８９．４％降至４．４％；抗ＧＰⅠｂ单抗ＨＩＰ１显著抑制ＣＡＡＶ活化的血小板表面ＧＭＰ１４０的表达，抑制程度与ＨＩＰ１浓度呈负相关（ｒ＝－０．９９２）。研究结果证明ＣＡＡＶ受体位于人血小板膜的ＧＰⅠｂ上。
ThelocalizationofChineseAgkistrodonAcutusvenom(CAAV)receptorsonplateletswasstudiedbyusingmonoclonalantibodyinhibitorytest,enzymatichydrolysisandradioimmunoassayofGMP140.TheresultsshowedthattwoantiplateletGPⅠbmonoclonalantibodies,SZ-2andHIP1,signifi-cantlyinhibitedthebindingof125I-CAAVtoplateletswithaconcentrationdependentmanner,themaxi-malinhibitionratebeing94.7%±2.0%and83.0%±2.9%,respectively.Withtheincreasingofthecon-centrationandthereactiondurationofchymotrypsin,thespecificbindingrateof125I-CAAVtoplateletsgradualydecreasedfrom89.4%to4.4%.MonoclonalantibodyagainstGPⅠb,HIP1,stronglyinhibitedtheGMP140expressiononCAAVactivatedplatelets,andtheGMP140expressioninverselycorrelatedwiththeconcentrationoftheantibody(r=-0.992).ItwasprovedthatCAAVreceptorsexistedinGPⅠbonplateletmembraneandCAAVexerteditsbiologicalefectbybindingtoGPⅠb.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
1996年第3期132-135,共4页
Chinese Journal of Hematology
关键词
中国蕲蛇毒
血小板受体
膜糖蛋白
ChineseAgkistrodonAcutusvenom(CAAV)PlateletreceptorMembranegly-coproteinMonoclonalantibody
