摘要
目的:研究雌激素受体(ER)在雄性大鼠肺组织的表达和定位。方法:采用RT-PCR方法检测大鼠肺组织ER-α、ER-βmRNA的表达,用免疫组织化学方法检测大鼠肺中ER-β蛋白表达定位。结果:RT-PCR结果显示,在大鼠肺组织中ER以ER-βmRNA的表达为主,ER-αmRNA几乎不表达。免疫组织化学检测结果显示ER-β存在于柱状上皮细胞、平滑肌细胞和间质细胞上,血管壁上内皮细胞和平滑肌细胞中也有表达。结论:ER-β在大鼠肺组织中有广泛的分布。
Objective To investigate the expression and distribution of the estrogen receptors (ER) in the lung of male rats. Methods The expressions of ER-α mRNA and ER-β mRNA in lung tissues was detected by reverse transcription-polymerase chain reaction(RT-PCR), and ER-β protein in lung was detected by immunohistochemistry. Results Aside from weak expression of ER-α mRNA, lung tissue showed moderate expression of ER-β mRNA. The positive ER-β immunoreactivity was presented in columnar epithelium, intermediate cells, basal ceils, smooth muscle ceils of lung tissue and in pulmonary vascular smooth muscle ceils, endothelial ceils. Conclusion ER-β is distributed in lung tissue of male rats.
出处
《东南大学学报(医学版)》
CAS
2005年第6期368-370,共3页
Journal of Southeast University(Medical Science Edition)
基金
铁道部科技基金资助项目(J2000Z086)
东南大学科学基金资助项目(9247341170)