摘要
体重２．５～３．０ｋｇ的日本大耳白兔，分为输精管结扎组（ＶＧ）和假手术对照组（ＳＯＧ）．术后４、８、１２、１８、２２ｍｏ经耳静脉采血，检测巨噬细胞（Ｍ）培养上清中白细胞介素１（ＩＬ－１）活性和外周血单个核细胞（ＰＢＭＣ）培养上清中白细胞介素２（ＩＬ－２）、白细胞介素６（ＩＬ－６）活性及血浆肿瘤坏死因子（ＴＮＦ）含量。结果表明：①ＩＬ－１活性和ＴＮＦ含量，术后第８ｍｏＶＧ均明显高于ＳＯＧ（Ｐ＜０．０２，Ｐ＜０．０１），术后４、８、１２、１８、２２ｍｏ的测定结果与ＳＯＧ比较均无明显差异（Ｐ＞０．０５）。②ＩＬ－２、ＩＬ－６活性，术后第８ｍｏＶＧ均明显低于ＳＯＧ（Ｐ＜０．０１，Ｐ＜０．０５），而术后第１２ｍｏ恢复到ＳＯＧ水平。③ＩＬ－２、ＩＬ－６活性间呈有意义的正相关关系（ｒ＝０．６６０，Ｐ＜０．００１）。
In this paper,we have observed dynamically the activities of IL-1,IL-2,and IL-6 from the culture supernate of peripheral blood and the contents of TNF in plasma in vasectomized rabbits.The results showed that:(1)the activity of IL-1 and the contents of TNF increased significantly in the rabbits vasectomized for 8 months(P<0.02,P<0.01)and recovered in the rabbits vasectomized for 12,18,22 months(P>0.05);(2)activities of IL-2,IL-6 were reduced remarkably in the rabbits vasectomized for 8 months(P<0.01,P<0.05)and returned to normal in the rabbits vasectomized for 12,18,22 months(P>0.05)respectively;and(3)the activities of IL-2 and IL-6 were significantly correlated(r=0.660,P<0.001).The present results may provide an experimental evidence for evaluating the functional changes of immunological cell in vasectomized animals.
出处
《免疫学杂志》
CAS
CSCD
北大核心
1996年第2期109-112,共4页
Immunological Journal
基金
国家"八五"科技攻关课题
