摘要
目的研究实验性癫痫大鼠在癫痫发作持续不同时间对海马神经元的影响.方法24只SD大鼠随机分成4组:诱发大鼠癫痫持续状态(status epilcpticus,SE)＜10、10～30、＞30 min组及正常对照组.于电镜下观察各组海马神经元超微结构变化;采用免疫组化方法检测凋亡相关基因bcl-2、bax的蛋白表达及通过流式细胞技术检测细胞凋亡情况.结果SE＜10 min组海马神经元所受影响不大,SE 10～30 min组海马神经元具有明显凋亡特征,SE＞30 min组多数海马神经元呈坏死性改变.结论大鼠SE对海马神经元损伤有凋亡和坏死两种不同形式,这与癫痫发作的持续时间密切相关.
Objective To study the effects on hippocampal neurons of epilepsy-induced rats with persistent various time courses of seizure. Methods 24 SD rats were divided randomly into 4 groups. Three groups of rats were status epilepticus （SE）-indueed and differed in seizure-lasting time as follow respectively SE〈10 min, SE 10-30 min and SE〉30 min. The fourth was a control group. The ultrastruetural morphological changes of hippoeampal neuronal cell were observed by electron microscopy, The apoptosis related gene Bcl-2 and Bax proteins in hippocampous were investigated by using immunochemical method and the apoptotie neurons were detected by flow eytometry. Results Little change was seen in the first group, but the hippoeampal neurons of the second group had significant apoptotie characters and the neurons showed necrotic features in the third. Conclusions SE triggers hippocampal neuronal degeneration comprised of both types necrosis and apoptosis, but which type is dominant depends on the duration of seizure activity.
出处
《中国神经免疫学和神经病学杂志》
CAS
2005年第6期350-352,356,共4页
Chinese Journal of Neuroimmunology and Neurology
关键词
癫痫
大鼠
海马神经元
epilepsy
rat
hippoeampal neuron
