摘要
目的研究血小板活化因子(PAF)受体拮抗剂海风藤酮治疗脑梗死的作用机制,并与传统的PAF受体拮抗剂银杏苦内酯相比较。方法采用磁共振成像与波谱技术原位动态观察海风藤酮对活体鼠脑组织损害程度、神经递质方面的影响。分别对缺血再灌注组及海风藤酮、银杏苦内酯治疗组鼠脑组织缺血、水肿及氮-乙酰天门冬氨酸(NAA)与乳酸(Lac)等代谢产物变化进行观察和比较。结果在缺血60m in再灌注1、3、6 h海风藤酮均能减小高信号区的体积,有效减少脑缺血后Lac/(PCr+Cr)比值的上升和NAA/(PCr+Cr)比值的下降,与银杏苦内酯治疗组差异无显著性(P>0.05),与缺血再灌组相比较差异有显著性(P<0.01)。结论海风藤酮与传统的PAF受体拮抗剂银杏苦内酯均具有明显的缺血后脑保护作用,磁共振成像与波谱技术可为缺血再灌注后神经脑保护剂作用机制的研究提供精确的神经影像学信息。
Aim To observe the neuroprotective effect of platelet activating factor receptor antagonist Kadsurenone on rat brain with cerebral ischemia/reperfusion. Methods Animal model was established through middle cerebral artery occlusion（MCAO）, we studied the changes of infarct volume, NAA and Lactate in Kadsurenone-treated group with DWI and ^1H- MRS examination after cerebral ischemia 60 min and reperfusion 1,3,6 h. Results Significant reductions in infarct volume were found in the Kadsurenone-treated group as well as in the Ginkgolides-treated group compared with the control group. And Kadsurenone decreased the concentration of lactate and prevented the decline of the concentration of NAA after cerebral ischemia/reperfusion. Conclusion As platelet-activating factor receptor antagonists, both Kadsurenone and Ginkgolides show remarkable neuroprotective effect. And MRI offers exact neuroimaging information for studying the neuroprotective meachnism of Chinese traditional medicine after cerebral ischemia and reperfusion.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2005年第11期1324-1327,共4页
Chinese Pharmacological Bulletin
基金
波谱与原子分子物理国家重点实验室资助项目(NoT152208)
