摘要
目的探讨基质金属蛋白酶-2(matrix metalloproteinase-2,MMP-2)和组织金属蛋白酶抑制物-2(tissue inhibi-tor of metalloproteinase-2,TIMP-2)在子宫内膜异位症发生发展中的作用。方法采用免疫组化链霉菌抗生物素蛋白-过氧化物酶染色法(S-P法)检测22例子宫内膜异位症的异位内膜和在位内膜组织中MMP-2、TIMP-2的表达,并以20例正常子宫内膜为对照组。结果在异位内膜组织的腺上皮细胞MMP-2呈高表达状态,与子宫内膜异位症在位内膜和正常子宫内膜相比,有非常显著性差异(P<0.01),TIMP-2在异位内膜组织中呈低表达,与子宫内膜异位症在位内膜和正常子宫内膜相比,差异有显著性(P<0.05),但MMP-2、TIMP-2在子宫内膜异位症在位内膜和正常子宫内膜中的表达,二者间没有显著性差别(P>0.05)。MMP-2、TIMP-2的表达强度与子宫内膜异位症的严重程度无明显相关性。结论异位内膜组织中MMP-2过度表达,而TIMP-2的表达下降,导致MMP-2/TIMP-2的比例增高,使异位内膜组织具有更强的侵袭力,可能在子宫内膜异位症的发生发展中起重要作用。
Objective To investigate the role of matrix metalloproteinase-2 （MMP-2） and tissue inhibitor of metalloproteinase-2 （ TIMP-2 ） in the pathogenesis of endometriosis. Methods Immunohistochemical S-P method was employed to detect the expression of MMP-2 and TIMP-2 in the ectopic tissues and eutopic endometrium of 22 patients with endometriosis and 20 normal controls. Results The expression of MMP-2 in glandular cells was significantly higher than those of uterine endometrium from women with and without endometriosis （P 〈0. 01 ）. The expression of TIMP-2 in the ectopic tissue was lower than that of the eutopic endometrium from women with endometriosis and the normal controls （P 〈 0. 05 ）. The expression of MMP-2 and TIMP-2 in eutopic endometrium showed no difference from that of the normal controls （ P 〉 0. 05 ）. The expressions of MMP-2 and TIMP-2 were not related to the degree of endometriosis. Conclusion The increased expression of MMP-2 and the decreased expression of TIMP-2 in the ectopic endometriotic tissues result in the higher ratio of MMP-2/TIMP-2 that can make ectopic endometrial tissues have a greater capacity to invade and play an important role in the pathogenesis of endometriosis.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2005年第21期2164-2166,共3页
Journal of Third Military Medical University
关键词
金属蛋白酶类
金属蛋白酶类组织抑制剂
细胞外基质
endometriosis
metalloproteinases
tissue inhibitor of metalloproteinase
extraceller matrix
