摘要
目的:观察雌孕激素替代治疗对去卵巢大鼠骨组织计量学参数变化及大鼠肾脏1,25-(OH)2D3受体(VDR)mRNA表达的影响,并探讨其可能的机制。方法:25只成年SD雌性大鼠,随机分为为5组,假手术组、单纯去势组以及去势+补充雌激素组、去势+补充孕激素组、去势+补充雌、孕激素组,每组5只。喂养3个半月后处死,取出肾脏,RT-PCR方法检测VDR mRNA的表达;取胫骨上段不脱钙骨制片,应用半自动图像数字化分析仪检测各组大鼠骨组织计量学参数。结果:与假手术组、雌激素及雌孕激素组相比,去卵巢大鼠松质骨骨量明显减少,伴有骨结构变差,骨形成和骨吸收的参数值增加(P<0.05);与去卵巢组相比,孕激素组大鼠无显著改变。去卵巢组大鼠肾脏VDR mRNA的相对表达量显著低于假手术组、雌激素组及雌孕激素组(P<0.05);而与孕激素组无显著差异。结论:雌激素能有效地抑制去卵巢大鼠的骨吸收和骨形成,降低骨转换,可能与促进去卵巢大鼠肾脏VDR mRNA的表达有关。
Objective：To study the influences of estrogen and progesteron on bone histomorphometry and renal expression of 1,25-dihydroxyvitamin D3 receptor（VDR） mRNA in ovariectomized rats. Methods： Twenty-five adult female SD rats were equally randomized into 5 groups：sham-operation （Sham）, ovariectomization（OVX）, ovariectomization plus estrogen treatment （OVX＋E）, ovariectomization plus progesteron treatment （OVX＋P） and ovariectomization plus both estrogen and progesteron （OVX＋E＋P） groups. All animals were killed to assess renal VDR mRNA expression by RT-PCR after 3.5 months feeding. Undecalcified proximal tibia was used to assess the bone histomorphometry with digitizing image analysis system. Results： OVX group, with weakened bone structure, had decreased cancellous bone and increased parameters of osteal formation and resorption compared with Sham, OVX＋E and OVX＋E＋P groups（P〈0.05）. The bone mass, osteal formation and resorption in group OVX＋P had no significant changes. VDR mRNA expression was markedly lower in OVX,OVX＋E and OVX＋E＋P groups than in Sham group（P〈0.05）. There was no significant changes of VDR mRNA expression in OVX＋P group. Conclusion： Estrogen can effectively prevent bone loss by inhibiting osteal formation and resorption and decreasing bone transformation in ovariectomized rats, which may be related to the increased transcription of renal VDR.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2005年第11期1270-1273,共4页
Academic Journal of Second Military Medical University
