摘要
目的观察雷公藤内酯醇对Ⅱ型胶原诱导的关节炎(CIA)大鼠外周血γδT细胞比率和凋亡的影响,探讨雷公藤治疗类风湿关节炎(RA)的可能机制。方法以胶原诱导的Wistar大鼠关节炎为动物模型,将造模成功的20只大鼠随机分为模型组和雷公藤内酯醇组。雷公藤内酯醇按4μg/100g体重肌肉注射给药,每3d1次,31d处死动物,运用直接免疫荧光法测定γδT细胞比率,流式细胞仪测定γδT细胞凋亡率。结果与正常对照组比较,CIA模型组外周血中γδT细胞比率明显下降,凋亡率升高,P<0.01;经雷公藤内酯醇治疗后,γδT细胞比率恢复,接近正常对照组,两组比较差异无统计学意义,P>0.05。结论减少γδT细胞凋亡,调节CIA大鼠外周血γδT细胞比率,可能是雷公藤内酯醇治疗RA的机制之一。目的观察雷公藤内酯醇对Ⅱ型胶原诱导的关节炎(CIA)大鼠外周血γδT细胞比率和凋亡的影响,探讨雷公藤治疗类风湿关节炎(RA)的可能机制。方法以胶原诱导的Wistar大鼠关节炎为动物模型,将造模成功的20只大鼠随机分为模型组和雷公藤内酯醇组。雷公藤内酯醇按4μg/100g体重肌肉注射给药,每3d1次,31d处死动物,运用直接免疫荧光法测定γδT细胞比率,流式细胞仪测定γδT细胞凋亡率。结果与正常对照组比较,CIA模型组外周血中γδT细胞比率明显下降,凋亡率升高,P<0.01;经雷公藤内酯醇治疗后,γδT细胞比率恢复,接近正常对照组,两组比较差异无统计学意义,P>0.05。结论减少γδT细胞凋亡,调节CIA大鼠外周血γδT细胞比率,可能是雷公藤内酯醇治疗RA的机制之一。
Objective To investigate the effects of triptolide on number of γδT lymphocyte and γδT lymphocyte apoptosis in peripheral blood of rat with collagen induced arthritis （CIA）. Methods The experimental Wistar rat model of CIA was set up successfully. The rats in triptolide group were administered with triptolide at 4 μg/100 g body weight intramuscularly every three days. The rats were sacrificed on the 31st day after the triptolide administration. The number of γδT lymphocyte was measured by direct fluoroimmunoassay. The γδT lymphocyte apoptosis in peripheral blood was analyzed by flow cytometry. Results Compared with normal group, the percentage of γδT lymphocyte in peripheral blood of CIA rat decreased, but the percentage of apoptotic γδT cells increased （P〈0.01）. After treatment with triptolide, the percentage of γδT lymphocyte in peripheral blood rose and the percentage of apoptotic γδT cells fell. There was significant difference in the percentage of γδT lymphocyte and apoptotic γδT cells between the triptolide group and the CIA group. Conclusion This study demonstrates that triptolide can reduce γδT lymphocyte apoptosis and modulate number of γδT lymphocyte in peripheral blood of CIA rat, which may be one of the mechanisms that triptolide treats rheumatoid arthritis.
出处
《中华风湿病学杂志》
CAS
CSCD
2005年第11期669-671,共3页
Chinese Journal of Rheumatology
基金
国家自然科学基金资助项目(30070961)
