摘要
目的:探讨非洲爪蟾VEGF肿瘤疫苗免疫抗小鼠纤维肉瘤的作用。方法:采用BALB/c小鼠建立MethA小鼠纤维肉瘤模型。实验肿瘤小鼠随机分成3个治疗组:非洲爪蟾VEGF(xVEGF)组、小鼠VEGF(mVEGF)组、生理盐水对照(NS)组。观察小鼠肿瘤生长、生存率和治疗的毒副反应,并检测抗自身mVEGF抗体、肿瘤微血管密度(microvesseldensity,MVD)。结果:xVEGF组肿瘤明显小于mVEGF组或NS对照组(P<0.05),xVEGF组小鼠生存率也明显高于mVEGF组或NS对照组(P<0.01)。WesternBlot检测发现异种蛋白质疫苗免疫的小鼠体内产生了抗自身mVEGF抗体,xVEGF组肿瘤MVD明显低于mVEGF组或NS对照组(P<0.01)。结论:非洲爪蟾VEGF疫苗治疗可诱导纤维肉瘤小鼠产生抗自身VEGF免疫反应及抗肿瘤作用。
Objective： To explore antitumor effect of immunotherapy with recombinant Xenopus laevis vascular endothelial growth factor （xVEGF） as a xenogeneic vaccine on fibrosarcoma in mice. Methods： Meth A fibrosarcoma model was established in BALB/c mice. These mice were randomly divided into three groups： xVEGF, mVEGF （mouse VEGF） and normal saline （NS） groups, and received relative treatments. Tumor growth, survival rate of tumor- bearing mice, and potential toxicity of the vaccines were investigated. Anti - mVEGF antibodies were detected by Western blot analysis. In addition, microvessel density （MVD） of tumor was detected by immunohistochemistry. Results： Tumor volumes of mice were significantly smaller in xVEGF group than in mVEGF or NS group （ P 〈 0.05）, and survival rate of mice was also significantly higher in xVEGF group than in mVEGF or NS group （ P 〈 0.01 ）. Anti - mVEGF antibodies were found by Western blot analysis in mice treated with xVEGF vaccine. Moreover, MVD in tumor tissues was significantly lower in xVEGF group than in mVEGF or NS group （ P 〈 0.01 ）. Conclusion： lmmanotherapy with xVEGF as a xenogeneic vaccine induces antitumor effect in mice by inducing immune response against self- VEGF.
出处
《华西医学》
CAS
2005年第4期696-697,共2页
West China Medical Journal
基金
国家重点基础研究发展计划(973计划)项目(2004CB518807)
科技部基础研究重大项目前期研究专项(国科基字[2001]50号)资助。
关键词
血管内皮细胞生长因子
异种肿瘤疫苗
免疫治疗
Vascular endothelial growth factor （VEGF）
Xenogeneic tumor vaccine
Immunotherapy
