摘要
目的观察高渗盐复合胶体液在失血性休克早期复苏中对小肠黏膜形态学特征的影响。方法36只SD大鼠随机分为三组,通过控制性颈动脉放血制成失血性休克模型,分别应用相同容量的乳酸钠林格氏液、7.5%高渗盐水和琥珀酸明胶的混合液、ATP-MgCl2-乳酸钠林格氏液进行液体复苏,复苏结束后2h处死动物,取末端回肠,常规固定切片染色,以光学图像分析法观察比较复苏后回肠黏膜的黏膜厚度和绒毛长度以及回肠黏膜上皮损伤指数等。结果三组复苏方案之间的回肠黏膜损伤指数比较有显著性差异(P<0.05),高渗盐明胶组的回肠黏膜损伤指数最小。三组复苏方案之间的回肠黏膜厚度和绒毛长度比较均无显著性差异。结论高渗盐复合胶体溶液在控制性失血性休克的早期复苏中对回肠黏膜的形态学损伤较小,能相对较好地保护肠黏膜物理屏障。
Objective To observe the morphological changes in intestinal mucosa after using 7.5 % hypertonic saline/ gelatin at early resuscitation after hemorrhagic shock. Methods Thirty-six SD rats were randomly divided into 3 groups. Hemorrhagic shock was induced by bloodletting in the artery to mean arterial pressure （MAP） of 4.67 to 5.33 kPa, and maintained for 60 minutes. The three groups were resuscitated with three different fluids （lactate Ringer＇s solution, 7.5 % hypertonic saline gelatin, ATP-MgCl2-1actate Ringer＇s solution）, respectively. The terminal ileums were harvested at 2 hours after resuscitation. The morphological changes in intestinal mucosa were observed under light microscope, including mucosal thickness, height of villi and mucosa damage index. Results Significant difference was found in mucosa damage index among the 3 groups （P 〈 0.05）. But there was no significant difference in mucosal thickness, height of villi among the 3 groups. Conclusion Hypertonic saline gelatin solution used at early resuscitation after hemorrhagic shock resuscitation is helpful in reducing mucosa damage in intestinal of the rats.
出处
《中华急诊医学杂志》
CAS
CSCD
2005年第11期922-924,共3页
Chinese Journal of Emergency Medicine
基金
上海市科学技术委员会基金资助项目(024119086)
