摘要
研究以凤仙花无菌苗胚芽段作为外植体,经过不含任何激素的MS(0)培养基预培养、MS+6-BA3mg/L+NAA0.5mg/L培养基的诱导芽和根分化、MS+6-BA1mg/L+NAA0.1mg/L培养基的诱导开花,在60d左右实现了凤仙花离体培养再生植株并在试管内开花.凤仙花离体培养试管内开花不仅具有一定的商业开发价值,而且为深入研究外界条件对植物离体开花的调控提供了一个简便的实验体系.
In the study, embryo bud of Impatiens halsamina L was cultured on hormone-free MS medium for promoting, MS medium with 6-BA 3 mg/L＋ NAA 0.5 mg/L for shoot and root formation, and MS medium containing 6-BA 3 mg/L＋NAA 0. 5 mg/L for flowering respectively. During about 60 days, plant regeneration and flowering of Impatiens haisamina L was finished in vitro. The result gave a clue for application of flowering of Impatiens baisamina L in vitro and developed a simple system for studying physiological mechanism of plant flowering.
出处
《杭州师范学院学报（自然科学版）》
CAS
2005年第4期293-294,共2页
Journal of Hangzhou Teachers College(Natural Science)
基金
浙江省自然科学基金项目(编号:Y304083)
关键词
凤仙花
组织培养
再生植株
开花
garden balsam（Impatiensbalsamina L）
tissue culture
plant regeneration
flowering
