摘要
目的探讨细胞间黏附分子-1(ICAM-1)及热休克蛋白70(HSP70)与猪同种异体胰腺移植排斥反应发生的关系。方法建立小型猪胰腺移植动物模型,通过移植胰腺穿刺活检组织学检查将标本分为Ⅰ(无排斥反应)、Ⅱ(轻度排斥反应)、Ⅲ(中度排斥反应)及Ⅳ(重度急性排斥反应)组。进一步行ICAM-1及HSP70免疫组织化学检查和图像分析。结果当移植胰腺发生排斥反应时,ICAM-1及HSP70的表达均升高,并随着排斥反应的加重而呈进行性升高。结论ICAM-1及HSP70均参与了胰腺排斥反应的发生过程,通过胰腺穿刺活检行ICAM-1及HSP70免疫组织化学检查可早期诊断排斥反应并判断排斥反应的严重程度。
Objective To investigate the relationships between the expressions of intercellular adhesion molecule 1 （ICAM-1）, heat shock protein 70（HSP70） and acute rejection after pancreas allotransplantation in miniature swine. Methods Ultrasound guided biopsies of the pancreas and histopathologic examination were performed after the miniature swine models of pancreas allotransplantation were established. According to the histological change, the samples were divided into group Ⅰ （no rejection）, group Ⅱ （mild acute rejection）, group Ⅲ（moderate acute rejection）and group Ⅳ （serious acute rejection）. The expressions of ICAM-1 and HSP70 were analyzed with immuno-histochemistry and imaging. Results The expressions of ICAM-1 and HSP70 were increased when acute rejection occurred after pancreas allotransplantation in miniature swine. The levels of ICAM-1 and HSP70 were sequentially high when acute rejection was serious. Conclusion ICAM-1 and HSP70 were involved in pancreas allograft rejection and useful for early diagnosis of acute rejection after pancreas transplantation.
出处
《中国医师杂志》
CAS
2005年第10期1340-1342,共3页
Journal of Chinese Physician
