摘要
The objective of this study was to determine the effects of ionomycin combined with cytochalasin B （CB）, cycloheximide （CHX）, or 6-dimethylaminopurine （6-DMAP） on the activation of porcine oocytes. In Experiment 1, in vitro matured oocytes were activated with 15, 20, 25 or 30 mmol L -1 ionomycin separately. Activation rates of 20, 25 mmol L-1 and 30 mmol L treatments were higher （P〈0.05） than that of 15 mmol L-1 treatment. In Experiment 2, in vitro matured oocytes were activated with 20 mmol L-1 ionomycin for 10, 20, 30, 40 or 50 rain and then incubated with 2 mmol L-1 6-DMAP for 6 h. Cleavage and blastocyst rates [（72.40 ± 13.02）%, （25.37 ± 11.43）%] after treatments for 40 min were higher （P〉0.05） than those of the other treatments. In Experiment 3, matured oocytes were activated with ionomycin and then incubated with 7.5 mg mL-1 CB, 10 mg mL-1 CHX, 2 mmol L-1 6-DMAP, 7.5 mg mL-1 CB ＋ 10 mg mL-1 CHX or 7.5 mg mL-1 CB ＋ 2 mmol L-1 6-DMAP for 6 h. The rates of activation, cleavage and blastocyst of 2 mmol L -1 6-DMAP treatment [（86.05 ± 4.29）%, （61.77 ±8.10）% and （21.62± 3.31）%] were higher （P〈0.05） than those of 7.5 mg mL-1 CB treatment. In Experiment 4, matured oocytes were activated with ionomycin and then incubated with 2 mmol L-1 6-DMAP for 3.5, 5.5 or 7.5 h. Cleavage rates and blastocyst rates of 5.5 h treatment [（66.59 ± 14.36）% and （25.40 ± 10.16）%] were higher （P〉0.05） than those of other treatments. In conclusion, activation of porcine oocytes appears to be most successful using the combination of ionomycin （20 mmol L-1, 40 min） followed by 6-DMAP （2 mmol L-1, 5.5 h）.
The objective of this study was to determine the effects of ionomycin combined with cytochalasin B （CB）, cycloheximide （CHX）, or 6-dimethylaminopurine （6-DMAP） on the activation of porcine oocytes. In Experiment 1, in vitro matured oocytes were activated with 15, 20, 25 or 30 mmol L -1 ionomycin separately. Activation rates of 20, 25 mmol L-1 and 30 mmol L treatments were higher （P〈0.05） than that of 15 mmol L-1 treatment. In Experiment 2, in vitro matured oocytes were activated with 20 mmol L-1 ionomycin for 10, 20, 30, 40 or 50 rain and then incubated with 2 mmol L-1 6-DMAP for 6 h. Cleavage and blastocyst rates [（72.40 ± 13.02）%, （25.37 ± 11.43）%] after treatments for 40 min were higher （P〉0.05） than those of the other treatments. In Experiment 3, matured oocytes were activated with ionomycin and then incubated with 7.5 mg mL-1 CB, 10 mg mL-1 CHX, 2 mmol L-1 6-DMAP, 7.5 mg mL-1 CB ＋ 10 mg mL-1 CHX or 7.5 mg mL-1 CB ＋ 2 mmol L-1 6-DMAP for 6 h. The rates of activation, cleavage and blastocyst of 2 mmol L -1 6-DMAP treatment [（86.05 ± 4.29）%, （61.77 ±8.10）% and （21.62± 3.31）%] were higher （P〈0.05） than those of 7.5 mg mL-1 CB treatment. In Experiment 4, matured oocytes were activated with ionomycin and then incubated with 2 mmol L-1 6-DMAP for 3.5, 5.5 or 7.5 h. Cleavage rates and blastocyst rates of 5.5 h treatment [（66.59 ± 14.36）% and （25.40 ± 10.16）%] were higher （P〉0.05） than those of other treatments. In conclusion, activation of porcine oocytes appears to be most successful using the combination of ionomycin （20 mmol L-1, 40 min） followed by 6-DMAP （2 mmol L-1, 5.5 h）.
