摘要
利用滴涂于铂盘表面的 Nation 膜中负电性的磺酸基与 anti-IgG 分子中的氨基阳离子之间的静电作用实现抗体的结合,同时通过负电性的纳米金增加抗体的固定量,最后在修饰电极的表面涂敷一层明胶(Gelatin)薄膜进行固定,制成 Gelatin/anti-IgG/Au/Nafion/Pt 膜,制得非标记免疫传感器.用循环伏安法(CV)和交流阻抗法(Nyquist)对电极逐层修饰过程进行了表征,并对免疫传感器的性能进行了研究.该传感器测定 IgG 的最低浓度为2.0μg/L,标准曲线的线性范围在5～960μg/L,回归方程为:ΔE=-2.2+31.7 log[IgG],响应时间为5 min.此传感器制备过程简单、稳定性好、灵敏度高、具有良好的选择性,用于生物样品分析,结果令人满意。
A highly sensitive potentiometrie immtmosensor has been developed for fast detection of human IgG based on Nation, gold nanoparticles and Gelatin as matrixes on platinum disk electrode. The assemble procedure was characterized by cyclic vohammetry and electrochemical impedance technique. The detection is based on the change of electric potential before and after the antigen - antibody reaction. Compared with the normal methods, this strategy could allow for antibody immobilized with a higher loading amount and better retained immunoactivity. As a model immunosensor, it successfully detected IgG in the concentration range 5 - 960μg/L with a detection limit of 2.0μg/L. Furthermore, there are several distinct advantages such as direct, rapid, simple and without multiple separation - labeling steps.
出处
《化学传感器》
CAS
2005年第3期47-52,共6页
Chemical Sensors
基金
西南大学高新技术培育基金(XSGX02)
西南师范大学博士基金(SWNUB2004021)
