摘要
利用紫外二阶导数光谱消除生物样品的背景吸收和部分合用药物对测定的干扰吸收，直接做血药浓度测定，建立血清中硫唑嘌呤浓度的测定方法。血清样品经乙酸乙酯提取后，氮气常压吹干，溶于甲醇０．２５ｍｌ中，以２７４ｎｍ和２９３ｎｍ为分析波长，峰谷法定量计算，硫唑嘌呤的最低检测浓度为２５ｎｇ·ｍｌ￣（－１），平均回收率为９９．３％±５．９％。方法简便、灵敏快速、干扰少。
A sensitive and rapid methed for the determination of azathiopurine concentrationin human serum was establiShed.By using ultraviolet second derivative spectra,the method eliminatedbackground interference of biological sample and coexisting drugs.The serum sample was extractedwith ethyl acetate,the extract was evaporated to dryness with a gentle N2 stream,and 0.25ml ofmethanol was added to disSolve the residue.Quantitative calculation was made with anal vticalwavelength of 274 nm and 293 nm,and peak-to- valley method.The minimum detectableconcentration of AZP in serum was 25 ng· ml-1 and average recovery was 99.3%±5.9%。
出处
《药学学报》
CAS
CSCD
北大核心
1996年第5期371-374,共4页
Acta Pharmaceutica Sinica
