摘要
为了解高钾抗心肌缺血再灌注（Ｉ－Ｒ）损伤的途径。用离体大鼠心脏行冠脉结扎，松扎后作电镜观察和磷酸盐－焦锑酸盐（ＰＰＡ法）细胞化学钙定位及抗氧自由基检测。结果：１３ｍｍｏ１／Ｌ高钾保护Ｉ－Ｒ心肌超氧化物歧化酶（ＳＯＤ）活力，抑制丙二醛（ＭＤＡ）生成，减少心肌酶的漏出，保存肌膜结合钙的能力，防止钙在线粒体的积聚，减轻心肌超微结构的破坏。实验提示１３ｍｍｏｌ／Ｌ钾能抑制胞内钙超负荷和膜脂质过氧化达到抗Ｉ－Ｒ损伤作用。
Aim:In order to investigate how hyperkalemia’protects myocardium agaenst is chemia and reperfusion(I-R)injury。Materials and Methods electron-microscopy,phosphate-pyroantimonate(PPA)calcium localizaton techniques and enzyme testing methods were used to investigate the changes in the isolated rat hearts。Results are as following:13 mmol/L potassium protects the activity of superoxide dismutase(SOD);reduces the content of malondialdehyde(MDA);decreases the release of lactate dehydrogenase(LDH);preserves membrane's ability to combine calcium;prevents Ca++ accumulatein in mitochondria(Mi)and alleviates cell ultrastructural injury。Conclusion:The results show that l3mmol/L potassium protects the myocardial I-R injury by depressing intracellular calcium overload and membrane lipid peroxidation.
出处
《中华胸心血管外科杂志》
CSCD
北大核心
1996年第3期179-181,共3页
Chinese Journal of Thoracic and Cardiovascular Surgery
