摘要
该文介绍了一种新的提取、纯化蓖麻毒素的方法,首次将p-苯胺基-1-巯基-β-D-吡喃半乳糖苷(p-aminobenzyl-1-thoi-β-D-galactopyranoside)琼脂糖凝胶作为亲和层析介质,结合凝胶层析介质Sephcaryl S-200,通过两步层析纯化,获得了高纯度的蓖麻毒素。MALDI-TOF测得分子量为62925,LD50为12.4μg/kg。该方法可以制备高纯度蓖麻毒素。
A new method for purifying ricin toxin from castor bean was presented, which used an affinity column and a gel chromatography column to obtain pure toxin from the crude extracts of castor beans. The use of Sepharose affinity matrix, p - aminobenzyl - 1 - thoi - β - D - galactopyranoside, was described first time.
出处
《生物技术》
CAS
CSCD
2005年第5期53-55,共3页
Biotechnology
关键词
蓖麻毒素
纯化
亲和层析
ricin toxin
punification
affinity chromatography
