摘要
目的观察亚低温对垂体后叶素所致实验性大鼠心肌缺血的影响,并探讨其发生机制。方法实验动物随机分成5组,每组10只,A组为常温缺血组;B组为常温硝酸甘油治疗组;C组为低温缺血组;D组为低温硝酸甘油治疗组;E组为对照组。结果A组心电图的阳性率最高,B组其次,E组最低,C组次低,D组介于B组与C组之间。低温C组的左心室功能较常温A组增加。比较A组、C组、E组的病理切片,E组正常,A组的阳性切片主要表现为心肌细胞疏松化,肌纤维呈颗粒状(早期缺血改变),C组以心外膜血管扩张充血,心肌细胞间水肿,局灶性心肌细胞疏松化为主。结论亚低温能减轻垂体后叶素所致的心肌损伤,改善左心室功能,对硝酸甘油的扩张冠状血管作用无明显影响。
Objectives To observe the effect of mild hypothermia on myocardial ischemia caused by Pituitrin, investigate the underlying mechanism. Methods 50 Wistar rats were randomized to five groups： bolus group E, myocardial ischernia group A with normothermia, myocardial ischernia group C with hypothermia, myocardial ischernia group B trea-ted with nitroglycerin under normal temperature, and myocardial ischernia group D treated with nitroglycerin under low temperature, each group has 10 rats. The A, C, E groups were treated with saline lml belly injecting; group B, D trea-ted with nitroglycerin （5mg/kg）. 30 minutes later pit （30U/kg） was given to each group except for the group E. Record the ECG, and LVSP ± dp/dtmax of group A and C. Core temperature was measured with thermistor. The mild hypothermia group received cooling under the body with the 4 ℃ saline bag. The core temperature was dropped to 32 - 35℃, while the control group＇s body temperature was kept above 37℃. afterwards, hearts were excised and cut to se-veral sections stained with HE for observing the degree of myocardial ischemia. Results 1. Comparison of the electrocardiogram negative rate. The negative rate of hypothermia group C was the most high for 8096 which was compared with A, B, D groups. The negative rate of group D treated wfith nitroglycerin under low temperature was the second. Group B treated with nitroglycerin under normal temperature was higher than group A without nitroglycerin. The difference was significant between group A and C（ P〈0.01 ）. There was difference as comparing group B and D with group A （ P〈0.05 ）. There was no difference between B and D, C and D group. 2. Heart function measurement after the treatment with pit, both of them deduced. Difference was significant between the two groups. The - dp/dtmax and LVSP had the same result. 3. HE Normothermia group A： cardiac myocyte became rarefaction, muscle fiber reported the grain form （earlier period of ischemia change）. Hypothermia group C： the v
出处
《中国心血管杂志》
2005年第5期332-335,共4页
Chinese Journal of Cardiovascular Medicine
关键词
亚低温
心肌缺血
垂体后叶素
Hypothermia
Myocardial ischemia
Pituitrin
