摘要
对10例经手术切除的进展期肿瘤病人用自体活化的肿瘤浸润淋巴细胞(TIL)和重组人白细胞介素-2(rhIL-2)治疗。治疗前1周及治疗后1个月检测细胞免疫功能,其中8例病人在治疗后PBL对同种异体相同组织学类型靶细胞的细胞毒性明显升高,而NK活性无明显变化;T_4、T_8荧光强度明显增强。10例病人对PHA、PPD反应性均明显增强。对人体无明显毒副作用。结果提示;TIL和rhIL-2治疗后可明显提两进展期肿瘤病人的细胞免疫功能,可能成为肿瘤术后重要的辅助治疗方法。
Tumor infiltrating lymphocytes (TILs) were isolated from 110 patients with advanced tumor. These cells were cultured in a complete medium supplemented with recombinant interleukin-2 (rIL-2) for 4-6 weeks. Cellular immunity response was assayed 1 week before and 1 month after TIL infusion. The results showed that cytotoxicity to same histological type target cells was much higher in the patients, there was no significant change in NK activity and there was a significant increase of fluorescent intensity in T_4, T_3 subset as compared with the background in 8 cases. PPD and PHA skin test were positive and the skin reactive lesion became larger when compared with background in 10 cases. 6 patients had transient mild chill and fecer, 2 cases had mild accompanying nausea and vomiting. The results indicate that TILs plus lower does rhL-2 were able to improve cellular immunity in patients with advanced tumor without causing severe side effect. It may play and important role in preventing recurrence and metastasis.
出处
《中华实验外科杂志》
CSCD
北大核心
1996年第2期82-83,共2页
Chinese Journal of Experimental Surgery
