摘要
目的:构建pCDNA3/p33ING1b真核表达质粒,探讨其对胃癌细胞株的生长抑制、凋亡的作用,探索新型的肿瘤治疗措施与方法。方法:构建pCDNA3/p33ING1b真核表达质粒,将p33ING1b转染至人胃癌细胞SGC-7901,研究其对胃癌细胞产生的作用。结果:重组pCDNA3/p33ING1b真核表达质粒构建成功,经p33ING1b转染的人胃癌细胞株SGC-7901生长速度减慢,融合时间变长,周期S期变短,G0/G1期延长,凋亡增加。结论:重组pCDNA3/P33ING1b质粒能在胃癌细胞SGC-7901细胞内表达,且能抑制胃癌细胞SGC-7901的生长并促进其凋亡。
Objective： To investigate the effective of constructing pCDNA3/p33^ING1b nuclear expressing microsome on growth inhibiting and apoptosis of stomach cancer cell line, and to search neotype antineoplastic measure. Methods. The pCDNA3/p33^ING1b nuclear expressing microsome was constructed, and p33^ING1b were implanted to human stomach cancer cell line（ SGC-7901 ）. The role of p33^ING1b to generate stomach cancer cell was studied. Results： The pCDNA3/p33^ING1b nuclear expressing microsome was constructed successfully. The human stomach cancer cell line （SGC-7901） under implantation of p33^ING1b showed a significant decrease in cell growth, the coupling time was delayed, DNA synthetic phase was shortened, G0/G1 phase was prolonged, and apoptosis was increased. Conclusion： Recombination pCDNA3/p33^ING1b nuclear expressing microsome, which expressed in stomach cancer cell line（ SGC-7901 ） , can inhibite the growth of stomach cancer cell line（ SGC-7901 ） , and promote its apoptosis.
出处
《江苏大学学报（医学版）》
CAS
2005年第5期415-417,共3页
Journal of Jiangsu University：Medicine Edition
