摘要
目的观察甲氧滴滴涕(MXC)对雄性小鼠的生殖系统毒性作用并探讨其毒性作用的可能机制。方法光镜观察喂服MXC小鼠睾丸和附睾的变化,并以生物化学的方法测定氧化损伤相关指标的改变。结果精子活动度和活率降低,畸形率增高,生精小管上皮变薄;睾丸和附睾的谷胱甘肽过氧化物酶(GSH-Px)活力和超氧化物歧化酶(SOD)活力显著下降(P<0.001),丙二醛(MDA)含量明显升高(P<0.01),维生素E组各项指标的变化均较21d组的变化小(P<0.05)。结论MXC对雄性小鼠生殖系统具有一定的毒性作用,可导致睾丸和附睾抗氧化能力下降,脂质过氧化增加,其毒性作用可被维生素E减轻。
[Objective] The aim of this study is to observe the toxic effect of MXC on genital system of male mice and to explore the possible toxic mechanism. [Methods] We observed the diversity of testis and epididymis of mice fed with MXC under microscope, and detected some indexes related to oxidative damage by using biochemistry methods. [Results] In the epididymis, the sperm activity and alive proportion rate decreased significantly, and the rate of malformation was raised. The spermatogenic epithelium was thinner than the controlled. The activities of GSH-PX and SOD in three MXC groups decreased significantly than the control group （P 〈0.001）, but the quantities of MDA were raised in comparison to the control group （P〈0.01）. In vitamin E group the change degree of each parameter was lighter than the group exposed to MXC 21 days （P〈0.05）. [Conclusions] MXC can act venomous effectiveness on reproductive system of male mice. MXC can decrease the capability of anti-oxidizing in testis and epididymis, and increase lipid peroxidation.VitE can weaken the effect of MXC.
出处
《中国医学工程》
2005年第5期466-469,共4页
China Medical Engineering
关键词
甲氧滴滴涕
氧化损伤
生精细胞
睾丸
methoxyehlor
oxidative damage
spermatogenic cell
testis
