摘要
目的观察大鼠骨髓微环境骨代谢相关基因表达变化,拟探讨外源性甲状旁腺激素(ParathyroidhormonePTH)治疗骨质疏松的分子机制。方法给予卵巢摘除(ovariectomyOVX)诱导的骨质疏松(OsteoporosisOP)大鼠每天20μgkg重组人甲状旁腺激素[rhPTH(134)]治疗8周,采用RTPCR方法检测大鼠骨髓细胞骨代谢相关基因的表达,比较PTH用药前、后及停药后各基因表达的变化。结果显示用药后骨髓细胞中成骨活性基因ALP、BGP、Cbfα1表达均持续显著升高(P<0.05～0.001);破骨细胞调节基因MCSF与RANKL表达变化无统计学意义;OPG与TRAF6表达呈双相波动(P<0.05～0.01);RANKLOPG比值在用药1周时增加(P<0.05);IL6表达呈早期短时升高(P<0.01)。结论PTH对骨质疏松的治疗作用可能与其持续增强骨髓细胞的成骨活性基因表达,调节破骨细胞分化和功能成熟基因表达有关。
Objective To investigate the molecular mechanism about the effects of parathyroid hormone（PTH） on OP.Methods Recombinant human parathyroid hormone（1-34） [ rhPTH（1-34） ] was subcutaneously injected once daily （20 μg/kg/d） for 8 weeks in model rats. RT-PCR was used to detect the gene expressions related with bone metablism in bone marrow stromal cells before and after the injection. Results The gene expressions of ALP, BGP and Cbfα1 in bone marrow stromal cells increased remarkably（ P 〈 0.05-0.001 ） after injected of PTH for l and 8 weeks, but no change was shown for M-CSF and RANKL. The expressions of OPG and TRAF-6 presented biphasically（ P 〈 0.05-0.01）.The ratio of RANKL/OPG increased at the 1st week post-injection（ P 〈 0.05）, and the expression of IL-6 increased transiently in early stage （ P 〈 0.01 ）. Conclusions The therapeutic effects of PTH on osteoporosis probably relate to the up-regulation of ALP, BGP and Cbfal gene expressions in bone marrow stromal cells, and the differentiation and mature of osteoclast.
出处
《中国骨质疏松杂志》
CAS
CSCD
2005年第3期286-288,共3页
Chinese Journal of Osteoporosis
