摘要
采用６－氨基喹啉－Ｎ－羟基琥珀酰亚胺基氨基甲酸酯（ＡＱＣ）为衍生剂，与各种氨基酸柱前定量衍生后，用ＷａｔｅｒｓＨＰＬＣ仪，ＡＱＣ－Ｔａｇ￣ＴＭＣ＿１８柱，以１４０ｍｍｏｌ／Ｌ从醋酸钠溶液（ｐＨ＝４．９５）为溶剂Ａ，乙腈－水（３：２）为溶剂Ｂ进行梯度洗脱，检测波长为２４８ｎｍ，以α－氨基丁酸为内标。所有１８种氨基酸在３７ｍｉｎ内测定完毕，各种氨基酸测定的重现性ＲＳＤ％均小于２．０％（ｎ＝５），平均回收率为９７．０％６～１０５．０％（ｎ＝５）。
The paper reports the determination of amino acids in compound amino acid injections using HPLC-AccQ-Tag method. All the amino acids reacted with 6-aminoquinoly-N-hydroxysuccinimdyl carbamate(AQC)respectively and quantitatively and produced corresponding derivatives which were analized by HPLC.Chromatographic conditions were AccQ-TagTM C18 for amino acids analysis(3.9mm×15cm),mobile phase of program elution (A)sodium acetate solution(pH=4.95)(B) acetonitrilewater(3:2);using α-aminobutyric acid as internal standard and detected at 248nm by UV-detector.The method was accurate,rapid,simple, convenint and high sensitive(×10-9mol). Eighteen kinds of amino acids were completely determined in 37 minutes. The correlation coefficients of the calibration graphs for seventeen amino acids were good (r≥0.9993), RSD of all amino acids were less than 2.0%and the average recoveries range were 97.0%～105.0%(n=5).
出处
《中国药科大学学报》
CAS
CSCD
北大核心
1996年第1期29-31,共3页
Journal of China Pharmaceutical University
关键词
氨基酸注射液
梯度洗脱
氨基酸
Compound amino acid injection
Derivative
Program elution
6-aminoquinolyl-N-hy-droxysuccinimidyl carbamate
