摘要
背景:转化生长因子β是一族多功能的蛋白多肽,在骨折愈合过程中起作用的主要为转化生长因子β1。不同的内固定材料对骨折愈合的影响不同,其影响程度如何是判定内固定材料优劣的一个重要评定指标。目的:通过不同内固定材料固定后骨折愈合不同阶段转化生长因子β1mRNA的表达,了解转化生长因子β1mRNA的细胞内定位,借此探讨不同内固定材料对骨折愈合过程中骨折局部转化生长因子β1mRNA的表达的影响。设计:随机对照观察。单位:解放军第二军医大学长海医院骨科、病理科。材料:实验于2000-03/2001-07在第二军医大学实验动物中心和骨科实验室完成。选用新西兰兔42只,所有动物均随机抽取,雌雄不限,按内固定物分成两组:矩形髓内钉组;不锈钢接骨板组,每组动物21只。方法:以矩形髓内钉与不锈钢接骨板固定兔胫骨制作骨折内固定模型,骨折内固定部位为兔左胫腓骨交界下2mm处。手术前后用市售动物颗粒饲料分笼喂养,饮用自来水,自由活动,不用外固定。每组动物于内固定手术后1,3,7,14,21,28d分别处死3只,取骨折端骨痂及周围组织标本,进行组织切片和原位杂交处理。主要观察指标:观测并记录组织切片中不同细胞内的转化生长因子β1mRNA染色阳性强度和阳性面积比,显示兔骨折愈合过程中骨痂中转化生长因子β1mRNA的分布和含量。结果:共用实验动物42只,腹泻死亡3只,伤口局部感染骨外露3只,共6只未纳入结果分析,矩形髓内钉组和不锈钢接骨板组各3只,进入结果分析动物数36只。①在骨折早期,矩形髓内钉组和不锈钢接骨板组局部的细胞和间质中转化生长因子β1mRNA均无表达;7d后间充质细胞、成骨细胞、成软骨细胞中有转化生长因子β1mRNA阳性表达。②矩形髓内钉组和不锈钢接骨板组转化生长因子β1mRNA阳性染色强度、阳性面积比及阳性指数术后14d最高(12.36±
BACKGROUND： Transforming group factor-beta （TGF-β） is a clan of protein peptide with multiple functions, of which, TGF-beta; plays the main role in union of hone fracture. The interfixation of various materials affects fracture union in different ways. The degree of influence is the important index to assay the quality of interfixation materials. OBJECTIVE： To understand intracellular orientation of TGFβ1mRNA by the expression of it at various phases of bone fracture union after interfixation with different materials so as to probe into the effects of different interrixation materials on the local expression of TGFβ1mRNA during fracture union. DESIGN： Randomized control observation was designed. SETTING： Department of Orthopedics and Department of Pathology of Changhai Hospital, Second Military Medical University of Cbinese PLA. MATERIALS： The experiment was performed irt Experimental Animal Center and Laboratory of Department of Orthopedics of the Second Military Medical University of Chinese PLA from March 2000 to July 2001, in which, 42 New Zealand rabbits were employed of either sex and randomized into two groups based on fixed materials, named as the group of rectangle-shaped intramedullary nail （RIN group） and group of stainless steel plate （SPL group）, 21 rahbits in each one. METHODS： Inteffixation model was prepared on rabbit tibia with either RIN or SPL, 2 mm inferior to the conjunction of tibia and fibula on the left side. Before and after operation, the rabbits were bred with market-sold animal granular forage in separate cages and they were free of drinking pipe water and movement, without external fixation. Three rabbits were sacririced in 1, 3, 7, 14, 21 and 28 days after inteffixation in each group suecessively. The callus on fractured end and peripheral tissue specimen were collected for managements of tissue slices and in situ hybridization. MAIN OUTCOME MEASURES： Positive staining intensity and positive area ratio of TGF β1mRNA in different cells were observed and r
出处
《中国临床康复》
CSCD
北大核心
2005年第34期181-183,共3页
Chinese Journal of Clinical Rehabilitation
