摘要
目的:观察碱性成纤维细胞生长因子对成骨细胞黏附性与成骨细胞DNA合成的影响。方法:实验于2004-08/12在广州军区广州总医院中心实验室完成。取2只健康新西兰大白兔骨髓基质细胞来源的成骨细胞体外培养。①光镜下观察成骨细胞形态。②进行不同浓度碱性成纤维细胞生长因子(0.1,1,10,100mg/L)诱导成骨细胞,设空白对照,于1,2,4,8h共4个时间点采用体视学计量黏附细胞数量。③流式细胞仪进行细胞DNA含量分析。结果:①成骨细胞特性观察:光镜下成骨细胞呈多角形或梭形表达出高碱性磷酸酶活性。②碱性成纤维细胞生长因子对成骨细胞黏附性的影响:碱性成纤维细胞生长因子浓度为1.0～10mg/L时细胞黏附数呈剂量依赖性,达到100mg/L时,细胞黏附数反而下降,时间到8h后,与对照组间无明显差异。③碱性成纤维细胞生长因子对成骨细胞DNA合成的影响:增殖指数显示碱性成纤维细胞生长因子浓度为1.0～10mg/L时呈剂量依赖性,到100mg/L时,指数略有下降,但仍显著高于对照组。结论:①碱性成纤维细胞生长因子浓度在1.0～10mg/L时可提高成骨细胞的黏附性。②碱性成纤维细胞生长因子浓度在1.0～10mg/L时可促进成骨细胞DNA合成,到100mg/L作用减弱。③碱性成纤维细胞生长因子可调节成骨细胞的黏附与增殖。
AIM： To study the effects of basic fibroblast growth factor （bFGF） on the adhesion and DNA synthesis of the osteoblasts. METHODS： The experiment was conducted at the central laboratory of General Hospital of Guangzhou Military Area Command of Chinese PLA between August and December 2004. The osteoblasts that were derived from the bone marrow stroma cells in 2 healthy New Zealand rabbit were cultured in vitro. ① The formation of osteoblast was observed under the light microscope. ② Different doses of bFGF （0.1,1,10,100 mg/L） were used to induce osteoblasts to built empty control. The number of adherent cells were calculated with stereology method at 1, 2, 4, 8 hours. ③ The DNA contents of the cells were analyzed with flow cytometer. RESULTS： ① Observation of osteoblast character： Under the light microscope the osteoblasts showed multiple angle or spindle shaped, and expressed high alkaline phosphatase activity. ② Effects of bFGF on the adherence of osteoblasts： The cell adherence showed dose-dependent effects when the concentration of bFGF was 1.0-10 mg/L; At 100 mg/L, the cell adherence decreased on the contrary; After the 8 hours, there was insignificant difference compared with the control group. ③ Effects of bFGF on the DNA synthesis of osteoblasts： The proliferation index showed dosedependent effects when the concentration of bFGF was 1.0-10 mg/L. At 100 mg/L the index decreased slightly, but still higher than that in the control group. CONCLUSION： ① The adherence of osteoblasts can enhance when the concentration of bFGF is between 1.0-10 mg/L. ② The DNA synthesis of osteoblasts is accelerated when the concentration of bFGF is between 1.0 -10mg/L, and the effects lighten at 100 mg/L. ③ The bFGF can adjust the adherence and proliferation of the osteoblasts.
出处
《中国临床康复》
CSCD
北大核心
2005年第34期40-42,i0003,共4页
Chinese Journal of Clinical Rehabilitation
基金
广东省医学科学技术研究基金(B2004100)~~
