摘要
背景:纳洛酮作为阿片受体的非特异性拮抗剂,已被用于治疗内源性阿片类物质异常释放有关的多种疾病。近年来的研究已经证实早期应用大剂量纳洛酮能够明显降低急性颅脑损伤患者的死亡率,促进神经功能恢复。目的:观察了盐酸纳洛酮在大鼠急性颅脑损伤实验模型中促进神经功能恢复的治疗作用,并做量效分析。设计:以实验动物为研究对象的随机区组设计。单位:北京市神经外科研究所。材料:取SD大鼠250只,随机分成6组:纳洛酮0.3,1.0,3.0,9.0mg/kg组,阳性对照组,阴性对照组。方法:采用Feenly自由落体撞击法建立颅脑损伤模型,于损伤后30min开始给药。前4组每天分别给予盐酸纳洛酮0.3,1.0,3.0,9.0mg/kg腹腔注射;阳性对照组给予胞磷胆碱钠2mg/只腹腔注射;阴性对照组给予0.5mL/只生理盐水腹腔注射,最长疗程为14d。主要观察指标:每天进行MNSS神经功能评分,伤后第2,4天每组随机取8只大鼠,通过干-湿重法计算脑组织的含水量。结果:250只大鼠中造模成功并进入结果分析172只。盐酸纳洛酮治疗组大鼠的神经功能恢复情况明显优于其他两组(P<0.01)。纳洛酮1,3,9mg/kg三组的情况优于0.3mg/kg组(P<0.05),而这三组之间没有显著性差异(P>0.05)。盐酸纳洛酮治疗组大鼠的脑含水量明显低于对照组(P<0.05);盐酸纳洛酮内部各实验组之间,0.3mg/kg组的脑含水量高于其他三组(P<0.05),其他三组之间无显著性差异(P>0.05)。结论:纳洛酮能够降低大鼠急性颅脑损伤后的脑水肿,对大鼠的神经功能恢复有明显的促进作用,并在一定范围内随着剂量的增加效果更显著。
BACKGROUND： As unspecific antagonist of opiate receptor, naloxone is widely used for multiple diseases which are related with abnormal release of endogenous opium. At present, researches suggest that large dosage of naloxone is used at early period can decrease death rate of patients with acute craniocerebral injury and promote neural functional recovery. OBJECTIVE： To investigate the effect of naloxone on improving the nervous function of rats with acute craniocerebral injury and to analyze effectively. DESIGN： Randomized grouping design based on the experimental animal. SETTING： Beijing Neurosurgical Institute. MATERIALS： Totally 250 SD rats were divided randomly into 0.3, 1.0, 3.0, 9.0 mg/kg naloxone group, positive control group and negative control group. METHODS： Craniocerebral injured model was established with Feenly free fall struck, and the medicine was given 30 minutes after injury. The rats of the first four experimental group were injected transpeniponeally with naloxone hydrochloride by 0.3 mg/kg, 1.0 mg/kg, 3.0 mg/kg and 9.0 mg/kg respectively once a day; meanwhile, the control groups were given 2 mg citicoline sodium for injection and 0.5 mL normal saline per rat respectively. The longest time was 14 days. MAIN OUTCOME MEASURES： MNSS neural functional score was used every day. The brain edemas of 8 rats in each group were measured with wet-dry weight methods on the second and the fourth day after head trauma. RESULTS： Among 250 rats, 172 entered the final analysis. The nervous function of rats in naloxone groups was better than the two control groups （P 〈 0.01）, and that in 1, 3 and 9 mg/kg naloxone group were better than 0.3 mg/kg group （P 〈 0.05）, but there were no significant differences a mong the three naloxone groups （P 〉 0.05）. The brain edemas of rats in naloxone groups were lighter than that in the control groups （P 〈 0.05）, and that of 1, 3 and 9 mg/kg groups were lighter than 0.3 mg/kg （P 〈 0.05）, but there were no significant differences am
出处
《中国临床康复》
CSCD
北大核心
2005年第37期162-163,共2页
Chinese Journal of Clinical Rehabilitation
