摘要
目的:研究人白介素-4(hIL-4)对经TNF-α活化的牛主动脉内皮细胞(BAEC)的保护作用。方法:用不同浓度的hIL-4与BAEC共孵育2h后,再与4μg/L的TNF-α共孵育6h或18h。应用细胞ELISA方法,检测BAEC表面的E选择素和ICAM-1的表达;用MTT比色法测定hIL-4对BAEC活性的影响。结果:在一定浓度内用hIL-4预处理BAEC后,能明显抑制TNF-α诱导活化BAEC上的E选择素与ICAM-1的表达,并呈现一定的剂量依赖性。用MTT比色法测定BAEC活性的实验表明,各实验组BAEC的活性与对照组无明显差异性。结论:hIL-4能明显抑制TNF-α诱导活化的BAEC表达E选择素与ICAM-1;hIL-4对BAEC的正常功能具有一定的保护作用。
AIM： To investigate the effect of human interleukin-4 （hIL-4） on the expression of E-selectin and ICAM-1 on bovine aortic endothelial cells（BAEC） activated with TNF-α, METHODS： BAEC were cultured in different concentrations of hIL-4 for 2 h followed by treatment with 4 μg/L TNF-α for 6 h or 18 h, The expression of E-selectin and ICAM-1 on BAEC was detected by cell-ELISA, The effect of hIL-4 on viability of BAEC was detected by MTT colorimetry. RESULTS： Pretreatment of BAEC with hIL-4 inhibited the expression of E-selectin and ICAM-1 on BAEC activated by TNF-α in a dose-dependent manner （ P 〈 0.01 ）. Both TNF-α and hIL-4 had no effect on the viability of BAEC. CONCLUSION： hIL-4 can suppress the expression of E-selectin and ICAM-1 on BAEC activated by TNF-α, which may contribute to the xenotransplant immune tolerance associated with hIL-4.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2005年第6期775-777,共3页
Chinese Journal of Cellular and Molecular Immunology
