摘要
种子细胞、支架材料及二者的相互作用是骨组织工程需要解决的三大问题。骨髓间充质干细胞(Mesenchym al stem cells,MSCs)是一种很有潜力的种子细胞,但挑选什么样的MSCs作为种子细胞目前研究尚少;而材料方面,研制力学性能和生物相容性均好的可降解多孔支架材料一直是研究者努力的方向。为了挑选处于最佳时期的种子细胞以及最适比例的β- TCP/PL L A多孔支架材料,我们观察并检测了大鼠MSCs(r MSCs)成骨诱导后不同时期细胞的形态及功能,发现r MSCs成骨诱导后10 d左右开始进入增殖期,14 d左右进入基质合成期,2 0 d左右进入矿化结节期(但三者不是截然分开的) ,从而根据实验目的挑选出能作为骨组织工程用的最佳细胞。将该时期的种子细胞与不同比例的β- TCP/PL L A多孔支架材料复合后,通过荧光显微镜、扫描电镜以及MTT等方法初步比较了不同比例的材料对细胞生长状况的影响,结果显示不同比例的材料均具有一定的生物相容性,细胞生长良好。但以β- TCP/PL L A=2∶1的材料最好,对细胞的生长影响最小。
There are three key factors in tissue engineering： seeding cells, scaffold and their interaction. Although mesenchymal stem cells（MSCs）are potential seeding cells,the problem of what phase MSCs should be used is not yet solved. On the other hand, degradable porous scaffolds which have good mechanics and good biocompatibility are preferred . To choose the optimum seeding cells and the suitable ratio of β-TCP/PLLA porous scaffold, we observed the phenotype of the male SD rat＇s osteoblastic MSCs and detected the amount of alkaline phosphatase,osteocalcin and type I collagen secreted by the osteoblastic rMSCs in different phase. About 10、14 and 20 days after induction, the induced cells came into proliferative phase, matrix synthesis phase and mineralization phase, respectively. Then we chose the suitable cells and seeded them on β-TCP/PLLA composite scaffolds with different ratios （β-TCP/PLLA = 1 ： 1; β-TCP/PLLA = 1 ： 2;and β-TCP/PLLA = 2 ： 1）. Fluorescence microscope,scanning electron microscope and MTT assay were used to observe and to detect the biocompatibility of the scaffolds. The results indicated that all of these materials have biocompatibility to some extent. Cells can grow well on all of the scaffolds. However, scaffold β-TCP/PLLA= 2 ： 1 seems to be a more suitable tissue engineering scaffold on account of its minimal influence on cell growth and differentiation.
出处
《生物医学工程学杂志》
EI
CAS
CSCD
北大核心
2005年第2期242-246,共5页
Journal of Biomedical Engineering
基金
国家高技术研究发展计划 (863计划 )资助项目 (2 0 0 2 AA3 2 60 80 )
