摘要
利用基因重组技术,人端粒酶催化亚单位基因反向插入真核表达载体pcDNA3.0,获得重组体pcDRTRT,通过脂质体法导入人结肠癌细胞株SW-111C,获得稳定转染细胞系,即反义细胞.该细胞易脱落,出现明显生长抑制现象;失去叠落生长能力;流式细胞仪(FCM)证实导入反义hTRT后,G0/1期细胞增加,G2M和S期细胞减少,增殖指数(PI)降低;且不能在软琼脂中形成集落.说明反义hTRT基因体外导入结肠癌细胞株SW-111C可以明显降低端粒酶活性,抑制结肠癌细胞的增殖且能使其恶性表型发生逆转.
hTRT was reversely inserted into pcDNA 3.0, and recombinant pcDRTRT was obtained. Recombinant pcDRTRT were transfected into colonic carcinoma cell line SW-111C with lipofectin and a stable transfected cell line. SW-111C-pcDRTRT was obtained. The transfected cells were charatered with easily dropping, slow growth and loss of overlap growth ability; Flow cytometry（FCM）detection showed that the cell in G0/1 phases of SW-lllC-pcDRTRT increased, but the cells in S and G2M phases decreased; The SW111C-pcDRTRT cells could not form in soft agar; the hTRT＇s expression of SW＇ 111C-pcDRTRT cells was significantly lower than that of control cells. The results indicated that telomerase activity of SW-lllC cells transfected with antise hTRT was decreased, cellular growth was inhibited and cellular malignant phenotype was reversed.
出处
《北华大学学报（自然科学版）》
CAS
2005年第5期406-408,共3页
Journal of Beihua University（Natural Science）
基金
吉林省教育厅科研基金项目(2003)
关键词
人端粒酶催化亚单位
人结肠癌细胞
反义基因治疗
Human telomerase reverse transcriptase
Colonic carcinoma cells
Antisense gene therapy
